Trigeminal P2X3 receptor expression differs from dorsal root ganglion and is modulated by deep tissue inflammation
Trigeminal P2X3 receptor expression differs from dorsal root ganglion and is modulated by deep tissue inflammation
复制标题
DOI：
10.1016/j.pain.2005.06.029
复制
发表时间：
2005-10-01
期刊：
影响因子：
7.4
通讯作者：
Dessem, D
中科院分区：
文献类型：
--
作者：
Ambalavanar, R;Moritani, M;Dessem, D
我们研究了P2X(3)受体在三叉神经节神经元中的分布和调节，以深入了解ATP在颅面感觉机制中的作用。在73%的P2X(3)阳性神经元中发现与d -半乳糖特异性凝集素1134结合，而只有16%的1134神经元表达P2X3。单独表达P2X3的神经元明显大于IB4或IB4/P2X(3)阳性神经元。靶特异性研究显示，22%的三叉神经节肌肉传入神经元P2X3阳性，16%的皮肤传入神经元P2X3阳性。肌肉P2X(3)传入神经元明显小于整体肌肉传入神经元群，而P2X(3)皮肤传入神经元则不是。假定的异质（P2X(2/3)）肌肉传入神经元也被确定，占P2X(3)肌肉传入神经元总数的77%。肌肉传入神经元与降钙素基因相关肽（15%）或P物质（4%）共表达P2X(3)。P2X(3)-阳性肌肉传入神经元的数量在完全弗氏佐剂诱导的咬肌炎症后1天和4天显著增加，但在12天后显著减少。这些结果表明，在三叉神经节内：(1)P2X(3)受体在中小神经元中均有表达；(2) P2X(3)受体在1134个神经元中不完全表达；(3) P2X(3)与神经肽共表达；(4)皮肤与肌肉P2X传入神经的比例差异不明显。因此，三叉神经P2X(3)神经元与背根神经节P2X(3)传入神经明显不同。该研究还表明，深层组织炎症调节P2X(3)受体的表达，因此可能值得探索作为治疗干预的靶点。(c) 2005年国际疼痛研究协会。Elsevier B.V.版权所有。
The distribution and modulation of the P2X(3) receptor was studied in trigeminal ganglion neurons to provide insight into the role of ATP in craniofacial sensory mechanisms. Binding to the D-galactose specific lectin 1134 was found in 73% of P2X(3)-positive neurons while only 16% of 1134 neurons expressed P2X3. Neurons expressing P2X3 alone were significantly larger than IB4-or IB4/P2X(3)-positive neurons. Investigation of target-specificity revealed that 22% of trigeminal ganglion muscle afferent neurons were positive for P2X3 versus 16% of cutaneous afferent neurons. Muscle P2X(3) afferents were significantly smaller than the overall muscle afferent population while P2X(3) cutaneous afferent neurons were not. Presumptive heteromeric (P2X(2/3)) muscle afferent neurons were also identified and comprised 77% of the P2X(3) muscle afferent population. Muscle afferent neurons co-expressed P2X(3) with either calcitonin gene-related peptide (15%) or substance P (4%). The number of P2X(3)-Positive muscle afferent neurons significantly increased one and four days following complete Freund's adjuvant-induced masseter muscle inflammation, but significantly decreased after 12 days. These results indicate that within trigeminal ganglia: (1) the P2X(3) receptor is expressed in both small and medium-sized neurons; (2) the P2X(3) receptor is not exclusively expressed in 1134 neurons; (3) P2X(3) is co-expressed with neuropeptides; (4) differences in the proportion of cutaneous versus muscle P2X(3) afferents are not apparent. Trigeminal P2X(3) neurons therefore differ markedly from dorsal root ganglion P2X(3) afferents. This study also shows that deep tissue inflammation modulates expression of the P2X(3) receptor and thus may warrant exploration as a target for therapeutic intervention. (c) 2005 International Association for the Study of Pain. Published by Elsevier B.V. All rights reserved.