Time-Resolved Observation of Product Ions Generated by 157 nm Photodissociation of Singly Protonated Phosphopeptides
Time-Resolved Observation of Product Ions Generated by 157 nm Photodissociation of Singly Protonated Phosphopeptides
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DOI：
10.1016/j.jasms.2009.08.021
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发表时间：
2009-12-01
影响因子：
3.2
通讯作者：
Reilly, James P.
中科院分区：
文献类型：
--
作者：
Kim, Tae-Young;Reilly, James P.
在光激发后300 ns到ms的时间范围内，记录了单电荷精氨酸终止的磷酸肽的真空紫外光解离串联质谱图，以研究磷酸基团何时从前体离子和产物离子上脱落以及串联质谱图中是否可以消除磷酸盐的损失。对于含有磷酸丝氨酸和磷酸苏氨酸的多肽离子，光激发后从产物离子中观察到的损失很小，最高可达1亩S。然而，光激活后仅300 ns，前体离子的中性损失就相当可观。产物离子的98Da损失最早出现在激光照射后约1lis处，在光激发后变得更为常见，约为13亩S。与以前的报道一致，磷酸酪氨酸比磷酸丝氨酸或磷酸苏氨酸更稳定。(J am Soc质谱学2009，20,2334-2341)(C)2009美国质谱学学会
Vacuum UV photodissociation tandem mass spectra of singly charged arginine-terminated phosphopeptides were recorded at times ranging from 300 ns to ms after photoexcitation, to investigate when the phosphate group falls off from the precursor and product ions and whether loss of phosphate can be eliminated in tandem mass spectra. For peptide ions containing phosphoserine and phosphothreonine, little loss of 98 Da from the product ions was observed up to 1 mu s after photoexcitation. However, neutral losses from the precursor ions were considerable just 300 ns after photoactivation. Loss of 98 Da from product ions first appears about 1 lis after laser irradiation and becomes more common 13 mu s after photoexcitation. Consistent with previous reports, phosphotyrosine was more stable than either phosphoserine or phosphothreonine. (J Am Soc Mass Spectrom 2009, 20, 2334-2341) (C) 2009 American Society for Mass Spectrometry