Effect of dietary meat and fish on endogenous nitrosation, inflammation and genotoxicity of faecal water
Effect of dietary meat and fish on endogenous nitrosation, inflammation and genotoxicity of faecal water
复制标题
DOI：
10.1093/mutage/gep070
复制
发表时间：
2010-05-01
期刊：
影响因子：
2.7
通讯作者：
Rodwell, Sheila A.
中科院分区：
文献类型：
--
作者：
Joosen, Annemiek M. C. P.;Lecommandeur, Emmanuelle;Rodwell, Sheila A.
N-3多不饱和脂肪酸与结肠肿瘤发生减少有关。然而，它们与结直肠癌发病率的关系尚不确定。我们调查了等热量的红肉脂肪鱼替代内源性亚硝化，炎症和遗传毒性的粪便水在明显健康的人类志愿者控制饮食的影响。14名志愿者分别食用高红肉、红肉/鱼组合和高鱼饮食8天。分析粪便匀浆中的血红素、亚硝基化合物（NOC）和钙卫蛋白以及相关上清液的遗传毒性。粪便NOC和血红素排泄量随着饮食中鱼的增加和肉的减少而减少。在所有日粮中，亚硝酰铁（FeNO）是总NOC的主要贡献者。其他NOC的比例增加，更多的鱼和更少的肉在饮食中（P = 0.01），导致在一个非统计学显着下降的比例FeNO的鱼的饮食。粪钙卫蛋白（P = 0.54）、粪水诱导的DNA链断裂、氧化嘌呤和氧化嘧啶在日粮间差异不显著（P > 0.36）。增加鱼的摄入量和减少红肉的摄入量似乎对炎症和粪便水诱导的（氧化）DNA损伤没有影响;然而，它确实减少了诱变和潜在致癌的NOC的形成，因此可能有益于影响结直肠风险。
N-3 polyunsaturated fatty acids have been associated with reduced colon tumorigenesis. However, their association with colorectal cancer incidence is not conclusive. We investigated the influence of isocaloric replacement of red meat with fatty fish on endogenous nitrosation, inflammation and genotoxicity of faecal water in apparently healthy human volunteers on controlled diets. Fourteen volunteers consumed a high red meat, a combined red meat/fish and a high fish diet for 8 days each. Faecal homogenates were analysed for haem, nitroso compounds (NOC) and calprotectin and associated supernatants for genotoxicity. Both faecal NOC and haem excretion decreased with more fish and less meat in the diet. Nitrosyl iron (FeNO) was the main contributor to total NOC on all diets. The proportion of other NOC increased with more fish and less meat in the diet (P = 0.01), resulting in a non-statistically significant decrease in the proportion of FeNO on the fish diet. There was no statistically significant difference in faecal calprotectin (P = 0.54) and faecal water-induced DNA strand breaks and oxidized purines and pyrimidines between the diets (P > 0.36). Increasing fish intake and reducing the intake of red meat does not seem to have an effect on inflammation and faecal water-induced (oxidative) DNA damage; however, it does reduce the formation of mutagenic and potentially carcinogenic NOC and may as such beneficially affect colorectal risk.