Carbohydrate binding activity of a lectin-like glycoprotein from stems and leaves of Dolichos biflorus.
Carbohydrate binding activity of a lectin-like glycoprotein from stems and leaves of Dolichos biflorus.
复制标题
双花豆茎叶中凝集素样糖蛋白的碳水化合物结合活性。
DOI：
10.1016/0006-291x(80)91185-7
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发表时间：
1980
影响因子：
3.1
通讯作者：
Borrebaeck,C
中科院分区：
文献类型：
--
作者：
Etzler,ME;Borrebaeck,C
已经发现，来自双花扁豆植物的茎和叶的糖蛋白与种子凝集素的抗体交叉反应，与共价连接到琼脂糖的血型A+ H物质的亲和柱结合。交叉反应性物质与亲和树脂的这种结合不同于种子凝集素的结合，因为它容易用0.15 M NaCl解离。使用包埋的血型A+ H物质的亲和电泳显示，交叉反应物质的碳水化合物结合活性被N-乙酰基-D-半乳糖胺和N-乙酰基-D-葡糖胺弱抑制。葡萄糖、甘露糖和半乳糖在50 mM浓度下测试时没有抑制作用。这些数据表明，交叉反应物质的特异性与种子凝集素的N-乙酰基-D-半乳糖胺特异性有些不同。这些发现的意义进行了讨论，在有关的交叉反应材料和种子凝集素的结构相似性。
A glycoprotein from the stems and leaves of the Dolichos biflorus plant that cross reacts with antibodies to the seed lectin has been found to bind to affinity columns of blood group A+ H substance covalently linked to Sepharose. This binding of the cross reactive material to the affinity resin differs from that of the seed lectin in that it is easily dissociated with 0.15 M NaCl. Affinity electrophoresis using entrapped blood group A+ H substance shows that the carbohydrate binding activity of the cross reactive material is weakly inhibited with N-acetyl-D-galactosamine and N-acetyl-D-glucosamine. Glucose, mannose and galactose gave no inhibition when tested at concentrations of 50 mM. These data indicate that the specificity of the cross reactive material is somewhat different from the N-acetyl-D-galactosamine specificity of the seed lectin. The significance of these findings is discussed in relation to the structural similarities of the cross reactive material and the seed lectin.