Proteomic approach to study the cytotoxicity of dioscin (saponin)
Proteomic approach to study the cytotoxicity of dioscin (saponin)
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DOI：
10.1002/pmic.200500595
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发表时间：
2006-04
期刊：
影响因子：
3.4
通讯作者：
Ying Wang;Y. Cheung;Zhiqi Yang;J. Chiu;C. Che;Qing‐Yu He
中科院分区：
文献类型：
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作者：
Ying Wang;Y. Cheung;Zhiqi Yang;J. Chiu;C. Che;Qing‐Yu He
从黄精（Polygonatum zanlanscianense pamp）的根中提取的薯蓣皂苷对人成髓细胞白血病HL - 60细胞具有细胞毒性。蛋白质组学分析显示，在HL - 60细胞中，线粒体相关蛋白的表达与薯蓣皂苷处理相对应，这表明线粒体是薯蓣皂苷的主要细胞靶点。线粒体功能研究证实，薯蓣皂苷处理可启动线粒体凋亡通路。除线粒体相关蛋白外，蛋白质组的变化暗示了其他机制也参与了diooscin诱导的HL - 60细胞凋亡，包括蛋白质合成活性损伤、细胞信号中磷酸酶的改变、氧化应激和细胞增殖的失调。目前对薯蓣皂苷处理HL - 60细胞中蛋白变化的研究表明，薯蓣皂苷通过多种诱导凋亡的途径发挥细胞毒性。
Dioscin, extracted from the root of Polygonatum zanlanscianense pamp, exhibits cytotoxicity towards human myeloblast leukemia HL‐60 cells. Proteomic analysis revealed that the expression of mitochondrial associated proteins was substantially altered in HL‐60 cells corresponding to the dioscin treatment, suggesting that mitochondria are the major cellular target of dioscin. Mitochondrial functional studies validated that mitochondrial apoptotic pathway was initiated by dioscin treatment. Changes in proteome other than mitochondrial related proteins implicate that other mechanisms were also involved in dioscin‐induced apoptosis in HL‐60 cells, including the activity impairment in protein synthesis, alterations of phosphatases in cell signaling, and deregulation of oxidative stress and cell proliferation. Current study of protein alterations in dioscin‐treated HL‐60 cells suggested that dioscin exerts cytotoxicity through multiple apoptosis‐inducing pathways.