IDENTIFICATION OF A PEPTIDE RECOGNIZED BY 5 MELANOMA-SPECIFIC HUMAN CYTOTOXIC T-CELL LINES
IDENTIFICATION OF A PEPTIDE RECOGNIZED BY 5 MELANOMA-SPECIFIC HUMAN CYTOTOXIC T-CELL LINES
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DOI：
10.1126/science.7513441
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发表时间：
1994-04-29
期刊：
影响因子：
56.9
通讯作者：
SLINGLUFF, CL
中科院分区：
文献类型：
--
作者：
COX, AL;SKIPPER, J;SLINGLUFF, CL
在由主要组织相容性分子HLA-A2. 1呈递的数千种肽中，至少9种被黑素瘤特异性细胞毒性T淋巴细胞（CTL）识别。串联质谱法用于鉴定和测序这些肽表位之一。黑色素瘤特异性CTL对这种9个残基的肽具有非常高的亲和力，该肽重建了来自测试的5个不同黑色素瘤患者中的每一个的CTL系的表位。通过多个CTL系的识别表明，这可能是用于基于肽的黑色素瘤疫苗的有希望的候选者。
Of several thousand peptides presented by the major histocompatibility molecule HLA-A2.1, at least nine are recognized by melanoma-specific cytotoxic T lymphocytes (CTLs). Tandem mass spectrometry was used to identify and to sequence one of these peptide epitopes. Melanoma-specific CTLs had an exceptionally high affinity for this nine-residue peptide, which reconstituted an epitope for CTL lines from each of five different melanoma patients tested. Recognition by multiple CTL lines suggests that this may be a promising candidate for use in peptide-based melanoma vaccines.