Applying TADF Emitters in Bioimaging and Sensing-A Novel Approach Using Liposomes for Encapsulation and Cellular Uptake.
Applying TADF Emitters in Bioimaging and Sensing-A Novel Approach Using Liposomes for Encapsulation and Cellular Uptake.
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DOI：
10.3389/fchem.2021.743928
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发表时间：
2021
影响因子：
5.5
通讯作者：
Pålsson LO
中科院分区：
文献类型：
--
作者：
Smith PO;Black DJ;Pal R;Avó J;Dias FB;Linthwaite VL;Cann MJ;Pålsson LO
开发了一种促进热激活延迟荧光（TADF）复合物的传递、摄取和细胞内定位的新方法。首先，证明了脂质体中TADF复合物的限制，其随后用作细胞摄取的递送载体。共聚焦荧光显微镜显示TADF复合物随后定位于HepG 2细胞的细胞质中。在这项工作中开发的程序包括在不破坏脂质体结构的情况下去除脂质体制剂中的分子氧。时间分辨荧光显微镜（点扫描）显示初始提示荧光，然后是弱的，但可检测的，延迟荧光成分的脂质体TADF内化在HepG 2细胞。通过证明可以递送未官能化和/或未屏蔽的TADF复合物，可以设想TADF（例如分子氧）的传感功能。
A new method for facilitating the delivery, uptake and intracellular localisation of thermally activated delayed fluorescence (TADF) complexes was developed. First, confinement of TADF complexes in liposomes was demonstrated, which were subsequently used as the delivery vehicle for cellular uptake. Confocal fluorescence microscopy showed TADF complexes subsequently localise in the cytoplasm of HepG2 cells. The procedures developed in this work included the removal of molecular oxygen in the liposome preparation without disrupting the liposome structures. Time-resolved fluorescence microscopy (point scanning) showed initial prompt fluorescence followed by a weak, but detectable, delayed fluorescence component for liposomal TADF internalised in HepG2 cells. By demonstrating that it is possible to deliver un-functionalised and/or unshielded TADF complexes, a sensing function for TADFs, such as molecular oxygen, can be envisaged.