Field evaluation of a rapid immunochromatographic dipstick test for the diagnosis of cholera in a high-risk population
Field evaluation of a rapid immunochromatographic dipstick test for the diagnosis of cholera in a high-risk population
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DOI：
10.1186/1471-2334-6-17
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发表时间：
2006-02-01
影响因子：
3.7
通讯作者：
Fournier, JM
中科院分区：
文献类型：
--
作者：
Wang, XY;Ansaruzzaman, M;Fournier, JM
背景：霍乱暴发的早期检测对于实施最适当的控制策略至关重要。方法：在2004年霍乱季节(1-5月)，在莫桑比克贝拉进行了一项前瞻性研究，评估了针对Oi霍乱弧菌的免疫层析试纸试验(法国巴黎巴斯德研究所)的性能。对391例急性水样非出血性腹泻患者的粪便标本进行试纸和常规培养。结果：与培养法比较，快速检测的敏感性和特异性分别为95%(91%可信区间：91%~99%)和89%(95%可信区间：86%~93%)。按样本类型(直肠拭子/大便)和腹泻严重程度分层后，敏感性在85%~98%之间，特异性在77%~97%之间。结论：在霍乱季节性暴发最迫切需要快速检测的环境中，这种一步试纸试验对霍乱弧菌的诊断具有良好的效果。
Background: Early detection of cholera outbreaks is crucial for the implementation of the most appropriate control strategies.Methods: The performance of an immunochromatographic dipstick test ( Institute Pasteur, Paris, France) specific for Vibrio cholerae OI was evaluated in a prospective study in Beira, Mozambique, during the 2004 cholera season ( January-May). Fecal specimens were collected from 391 patients with acute watery nonbloody diarrhea and tested by dipstick and conventional culture.Results: The overall sensitivity and specificity of the rapid test compared to culture were 95% ( 95% confidence interval [CI]: 91% - 99%) and 89% ( 95% CI: 86% - 93%), respectively. After stratification by type of sample ( rectal swab/bulk stool) and severity of diarrhea, the sensitivity ranged between 85% and 98% and specificity between 77% and 97%.Conclusion: This one-step dipstick test performed well in the diagnosis of V. cholerae OI in a setting with seasonal outbreaks where rapid tests are most urgently needed.