The expression and distribution of immunomodulatory proteins B7-H1, B7-DC, B7-H3, and B7-H4 in rheumatoid synovium
The expression and distribution of immunomodulatory proteins B7-H1, B7-DC, B7-H3, and B7-H4 in rheumatoid synovium
复制标题
类风湿滑膜中免疫调节蛋白B7-H1、B7-DC、B7-H3和B7-H4的表达和分布
DOI：
10.1007/s10067-011-1815-1
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发表时间：
2012-02-01
影响因子：
3.4
通讯作者：
Chen, Yongwen
中科院分区：
文献类型：
--
作者：
Guo, Guoning;Shang, Yongjun;Chen, Yongwen
CD28/B7 信号已被证明具有调节 T 细胞活化并参与调节类风湿性关节炎 (RA) 发展的能力。然而，B7超家族的一些成员（包括B7-H1、B7-DC、B7-H3和B7-H4）在RA滑膜中的表达和解剖分布仍不清楚。我们分析了这些分子在 RA 患者滑膜组织中的表达。免疫组织化学显示在滑膜中观察到所有这些分子。在细胞水平上，它们全部存在于细胞膜和细胞质中。 B7-DC和B7-H3主要表达于毛细血管、滑膜细胞和内衬层浸润炎症细胞，而B7-H1和B7-H4在部分亚衬和内衬层炎症细胞中表达。荧光双重染色表明所有这些分子主要与CD31+内皮细胞和CD68+巨噬细胞相关。此外，在CD3+T细胞（包括CD4+和CD8+T细胞）上也观察到B7-H1和B7-H3。有趣的是，B7-H1/B7-H4、B7-H3/B7-DC 在同一细胞上共表达。这些分子在滑膜中的特征性表达和分布表明它们可能在RA的进展过程中发挥不同的作用，清楚地了解它们的功能作用可能会进一步阐明该疾病的发病机制。
CD28/B7 signals have been shown to have the capacity to regulate T cell activation and participate in regulating the development of rheumatoid arthritis (RA). However, the expression and anatomical distribution of some members of the B7 superfamily including B7-H1, B7-DC, B7-H3 and B7-H4 in RA synovium is still unclear. We analyzed the expression of these molecules in synovial tissues from RA patients. Immunohistochemistry showed that all of these molecules were observed in synovium. On the cellular level, all of them were found on cell membrane and in cytoplasma. The expression of B7-DC and B7-H3 was major on capillaries, synovicytes and infiltrated inflammatory cells in the lining layer, while B7-H1 and B7-H4 were detected in some inflammatory cells residing in the sublining and lining layer. Fluorescent dual staining indicated that all these molecules were principally associated with CD31+endothelial cells and CD68+macrophages. In addition, B7-H1 and B7-H3 were also observed on CD3+T cells (including CD4+and CD8+T cells). Interestingly, B7-H1/B7-H4, B7-H3/B7-DC were co-expressed on the same cells. The characteristic expression and distribution of these molecules in synovium indicated that they probably have different effects during the progress of RA, and a clear understanding of their functional roles may further elucidate the pathogenesis of this disease.