A new approach to detection of the bregma point on the rat skull
A new approach to detection of the bregma point on the rat skull
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DOI：
10.1016/j.jneumeth.2009.09.022
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发表时间：
2010-01-15
影响因子：
3
通讯作者：
Lewandowski, Marian H.
中科院分区：
文献类型：
--
作者：
Blasiak, Tomasz;Czubak, Wojciech;Lewandowski, Marian H.
立体定向通常用于在体内将微电极或微探针植入大脑的特定结构中。在这种技术中，脑核的位置被确定为与颅骨上定义的参考点的距离。因此，正确定位参考点至关重要。在啮齿动物的头盖骨上，主要的立体定位参考点被称为前囟，并被定义为沿冠状缝沿着最佳拟合曲线的中点。粗略地确定前囟的位置常常会导致错误。在我们的实验中，我们开发并测试了一种替代方法，定位成熟Wistar大鼠头骨上的前囟点。在该方法中，通过计算机分析暴露的颅骨帽的数字图像。该曲线在数学上与冠状缝的轮廓拟合，并且基于颅骨的颞脊描绘脑中线。这两条线的交叉被定义为前囟。对这种新方法的系统性实验测试表明，在许多情况下，通过两种不同的方法（旧的，粗略的方法和新的方法）定位的前囟点的位置变化多达数百微米。当使用新的方法确定前囟时，微探针在大脑中的立体定位误差显着降低。这些结果证实了定位立体定位参考点的新方法提高了体内电极植入的精度。(C)2009爱思唯尔有限公司版权所有。
Stereotaxy is commonly used to implant microelectrodes or microprobes in specific structures of the brain in vivo. In this technique, the positions of the brain nuclei are determined as the distance from a defined reference point on the skull. Thus, it is crucial to correctly locate the reference point. On the rodent skull cap, the principal stereotaxic reference point is called the bregma and is defined as the midpoint of the curve of best fit along the coronal suture. Rough determination of the position of the bregma often results in error. In our experiments we developed and tested an alternative method of locating the bregma point on the skull of mature Wistar rats. In this method, a digital picture of the exposed skull cap is analyzed by a computer. The curve is mathematically fitted to the outline of the coronal suture, and the brain midline is delineated based on the temporal ridges of the skull. The crossing of these two lines is defined as the bregma. Systematic, experimental testing of this new method revealed that, in many cases, the position of the bregma point as located by two different methods (old, rough method and the new one) varied by as much as hundreds of microns. The error in stereotaxic positioning of the microprobe in the brain was significantly decreased when the bregma was determined using the new approach. These results confirm that the new method of locating the stereotaxic reference point improves the precision of in vivo electrode implantation. (C) 2009 Elsevier B.V. All rights reserved.