Requirement for Nuclear Calcium Signaling in Drosophila Long-Term Memory
Requirement for Nuclear Calcium Signaling in Drosophila Long-Term Memory
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DOI：
10.1126/scisignal.2003598
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发表时间：
2013-05
影响因子：
7.3
通讯作者：
Jan-Marek Weislogel;C. P. Bengtson;Michael Müller;J. N. Hörtzsch;M. Bujard;C. Schuster;H. Bading
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文献类型：
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作者：
Jan-Marek Weislogel;C. P. Bengtson;Michael Müller;J. N. Hörtzsch;M. Bujard;C. Schuster;H. Bading
阻断核钙信号可逆地抑制果蝇长期记忆的形成。记忆的形成是一个复杂的过程，长期记忆的形成需要基因表达的变化来持续适应神经元回路。创造与气味相关的令人厌恶的经历的持久记忆的能力可能提供了生存优势。Weislogel等人使用具有遗传编码的钙传感器和蛋白质的果蝇，这些蛋白质特异性地干扰细胞核而不是细胞溶质的钙信号，以在体内显示细胞核钙对于长期嗅觉回避记忆形成的变化是特异性需要的，但对于不需要基因表达的其他形式的嗅觉回避记忆则不是。这项研究提供了一个演示的信号所需的持久的记忆形成在体内。钙在整个进化过程中被用作细胞内的信号传导器。在哺乳动物中枢神经系统中，钙介导突触和细胞核之间的对话，这是转录依赖性持续神经元适应所必需的。在无脊椎动物大脑中，核钙信号在类似过程中的作用还有待研究。在这里，我们表明，在体内钙成像的成年果蝇黑腹果蝇脑神经元，电脚休克用于嗅觉回避条件诱发短暂增加细胞质和核钙离子浓度的神经元。这些钙信号是在苍蝇蘑菇体的凯尼恩细胞中检测到的，这些细胞是与气味相关的学习和记忆部位。急性阻断核钙信号在条件反射选择性和可逆地取消了长期嗅觉回避记忆的形成，而短期，中期，或麻醉耐受性嗅觉记忆保持不受影响。因此，果蝇的记忆从不稳定到转录依赖的持久形式的进展需要核钙信号。这些结果确定核钙作为一种进化上保守的信号需要在无脊椎动物和脊椎动物的大脑转录依赖的记忆巩固。
Blocking nuclear calcium signaling reversibly inhibits long-term memory formation in flies. Flies Need Nuclear Calcium to Remember Memory formation is a complex process, and the formation of long-term memories requires changes in gene expression to persistently adapt the neuronal circuitry. The ability to create lasting memories of aversive experiences associated with smells likely provides a survival advantage. Weislogel et al. used fruit flies with genetically encoded calcium sensors and proteins that interfered specifically with the nuclear, but not cytosolic, calcium signals to show in vivo that nuclear calcium was specifically required for changes in long-term olfactory avoidance memory formation, but not for other forms of olfactory avoidance memories that do not require gene expression. This study provides a demonstration of the signals required for lasting memory formation in vivo. Calcium is used throughout evolution as an intracellular signal transducer. In the mammalian central nervous system, calcium mediates the dialogue between the synapse and the nucleus that is required for transcription-dependent persistent neuronal adaptations. A role for nuclear calcium signaling in similar processes in the invertebrate brain has yet to be investigated. Here, we show by in vivo calcium imaging of adult brain neurons of the fruit fly Drosophila melanogaster, that electrical foot shocks used in olfactory avoidance conditioning evoked transient increases in cytosolic and nuclear calcium concentrations in neurons. These calcium signals were detected in Kenyon cells of the flies’ mushroom bodies, which are sites of learning and memory related to smell. Acute blockade of nuclear calcium signaling during conditioning selectively and reversibly abolished the formation of long-term olfactory avoidance memory, whereas short-term, middle-term, or anesthesia-resistant olfactory memory remained unaffected. Thus, nuclear calcium signaling is required in flies for the progression of memories from labile to transcription-dependent long-lasting forms. These results identify nuclear calcium as an evolutionarily conserved signal needed in both invertebrate and vertebrate brains for transcription-dependent memory consolidation.