A three-dimensional collagen scaffold cell culture system for screening anti-glioma therapeutics.
A three-dimensional collagen scaffold cell culture system for screening anti-glioma therapeutics.
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用于筛选抗神经胶质瘤治疗药物的三维胶原支架细胞培养系统
DOI：
10.18632/oncotarget.10885
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发表时间：
2016-08-30
期刊：
影响因子：
--
通讯作者：
Yao XH
中科院分区：
文献类型：
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作者：
Lv D;Yu SC;Ping YF;Wu H;Zhao X;Zhang H;Cui Y;Chen B;Zhang X;Dai J;Bian XW;Yao XH
三维（3D）培养，可以模拟体内微环境，已越来越多地用于研究肿瘤细胞生物学。由于大多数临床前抗胶质瘤药物试验仍然依赖于传统的2D细胞培养，我们建立了用于3D胶质瘤细胞培养的胶原支架。在这些三维支架上培养的胶质瘤细胞比在二维培养中的细胞表现出更大程度的去分化和静止。3D培养的细胞也表现出对化疗烷化剂的抗性增强，胶质瘤干细胞的比例高得多，O 6-甲基鸟嘌呤DNA甲基转移酶（MGMT）上调。重要的是，3D培养中的肿瘤细胞显示出与胶质瘤患者相似的化疗耐药模式。我们的研究结果表明，三维胶原支架是有前途的体外研究平台，筛选新的抗胶质瘤治疗。
Three-dimensional (3D) culture, which can simulate in vivo microenvironments, has been increasingly used to study tumor cell biology. Since most preclinical anti-glioma drug tests still rely on conventional 2D cell culture, we established a collagen scaffold for 3D glioma cell culture. Glioma cells cultured on these 3D scaffolds showed greater degree of dedifferentiation and quiescence than cells in 2D culture. 3D-cultured cells also exhibited enhanced resistance to chemotherapeutic alkylating agents, with a much higher proportion of glioma stem cells and upregulation of O6-methylguanine DNA methyltransferase (MGMT). Importantly, tumor cells in 3D culture showed chemotherapy resistance patterns similar to those observed in glioma patients. Our results suggest that 3D collagen scaffolds are promising in vitro research platforms for screening new anti-glioma therapeutics.