Development of a human milk protein standard.
Development of a human milk protein standard.
复制标题
制定人乳蛋白质标准。
DOI：
10.1111/j.1651-2227.1989.tb11053.x
复制
发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Lönnerdal,B
中科院分区：
文献类型：
--
作者：
Donovan,SM;Lönnerdal,B
母乳 (HM) 蛋白质含量的准确定量对于确定母乳喂养婴儿的蛋白质和能量摄入量具有临床重要意义。蛋白质可以通过凯氏定氮分析或比色测定来测定。比色测定快速且方便，但与凯氏定氮蛋白 (KP) 相比，通常会高估蛋白质含量。在本研究中，通过尺寸排阻柱色谱法分离出基于人乳蛋白 (HMPS) 的蛋白质标准品。标准品的纯度和组成通过凝胶电泳、凯氏定氮法和氨基酸分析来确定。使用牛血清白蛋白、人血清白蛋白/IgG 和 HMPS 作为标准品，通过 Lowry、BCA 和 BioRad 比色法测定 20 个成熟奶样品的蛋白质含量。使用 Student 的 f 检验将这些结果与 KP 结果进行比较。所有比色分析都高估了牛奶蛋白质含量；然而，Lowry 测定给出的蛋白质水平最低，我们的蛋白质标准产量值最接近 KP 值。
Accurate quantitation of the protein content of human milk (HM) is clinically important for both determining protein and energy intakes of HM‐fed infants. Protein can be determined by Kjeldahl analysis or colorimetric assays. Colorimetric assays are rapid and convenient, but usually overestimate the protein content when compared to Kjeldahl protein (KP). In this study a protein standard based on human milk protein (HMPS) was isolated by size exclusion column chromatography. Purity and composition of the standard was determined by gel electrophoresis, Kjeldahl and amino acid analyses. The protein content of 20 mature milk samples was determined by the Lowry, BCA and BioRad colorimetric assays, using bovine serum albumin, human serum albumin/IgG, and HMPS as standards. These results were compared with KP results using Student's f‐test. All colorimetric assays overestimated milk protein content; however, the Lowry assay gave the lowest protein levels, with our protein standard yielding values closest to the KP value.