Calcium and Magnesium Supplementation Improves Serum OPG/RANKL in Calcium-Deficient Ovariectomized Rats
Calcium and Magnesium Supplementation Improves Serum OPG/RANKL in Calcium-Deficient Ovariectomized Rats
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DOI：
10.1007/s00223-010-9410-z
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发表时间：
2010-10-01
影响因子：
4.2
通讯作者：
Kim, Mi-Hyun
中科院分区：
文献类型：
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作者：
Bae, Yun Jung;Kim, Mi-Hyun
据报道，镁（Mg）缺乏可通过细胞因子系统的变化导致骨吸收增加，如血清骨保护素（OPG）浓度降低和NF-κ B配体受体激活剂（RANKL）浓度升高。然而，关于镁补充对OPG和RANKL的影响的数据很少。本研究旨在探讨补充镁对去卵巢大鼠骨密度（BMD）、骨矿物质含量（BMC）、血清OPG和RANKL的影响。将55只Sprague-Dawley雌性大鼠分为以下5组，并按所示饲喂12周：假手术对照组（sham）、OVX缺钙组（OLCa，0.1%Ca和0.05%Mg），OVX缺钙和补充Mg组（OLCaMg，0.1% Ca和0.1% Mg）、OVX钙充足组（OACa，0.5% Ca和0.05% Mg）和OVX钙充足镁补充组（OACaMg，0.5% Ca和0.1% Mg）。OVX组腰椎、股骨和胫骨的BMD显著低于Sham组。与低钙饮食组相比，无论是否补充镁，具有充足钙饮食的OVX组腰椎BMC均显着较高。OACaMg组的OPG水平和OPG/RANKL比率显着高于OLCa组。从上述结果来看，目前尚不清楚镁补充是否可以改善骨矿物质状态，而镁补充与充足的钙饮食导致细胞因子的变化，可能促进骨形成。
Magnesium (Mg) deficiency has been reported to result in increases in bone resorption through changes in the cytokine system, such as decreases in serum osteoprotegerin (OPG) concentrations and increases in receptor activator of NF-kappa B ligand (RANKL) concentrations. However, there are few data about the effects of Mg supplementation on OPG and RANKL. This study was carried out to investigate the effects of Mg supplementation on bone mineral density (BMD), bone mineral content (BMC), serum OPG, and RANKL in ovariectomized (OVX) rats relative to calcium (Ca) intake levels. Fifty-five Sprague-Dawley female rats were divided into the following five groups and fed for 12 weeks as indicated: sham-operated control group (sham), OVX Ca-deficient group (OLCa, 0.1% Ca and 0.05% Mg), OVX Ca-deficient and Mg-supplemented group (OLCaMg, 0.1% Ca and 0.1% Mg), OVX Ca-adequate group (OACa, 0.5% Ca and 0.05% Mg), and OVX Ca-adequate and Mg-supplemented group (OACaMg, 0.5% Ca and 0.1% Mg). The BMD of the lumbar spine, femur, and tibia in the OVX groups was significantly lower than that in the sham group. The OVX group with an adequate-Ca diet showed significantly higher BMC of the lumbar spine compared to the low Ca-diet group regardless of Mg supplementation. The OACaMg group had significantly higher levels of OPG and OPG/RANKL ratio than did the OLCa group. From the above results, it is still unclear whether Mg supplementation can improve bone mineral status, while Mg supplementation with an adequate-Ca diet resulted in a change in cytokines that may promote bone formation.