Bioactivity and metabolism of trans-resveratrol orally administered to Wistar rats
Bioactivity and metabolism of trans-resveratrol orally administered to Wistar rats
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DOI：
10.1002/mnfr.200500003
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发表时间：
2005-05-01
影响因子：
5.2
通讯作者：
Somoza, V
中科院分区：
文献类型：
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作者：
Wenzel, E;Soldo, T;Somoza, V
本研究的目的是探讨白藜芦醇在体内选择的化学预防参数和代谢转化的影响。在两个为期8周的大鼠喂养实验中，给予低白藜芦醇饮食（每公斤体重50毫克白藜芦醇）和高白藜芦醇饮食（每公斤体重300毫克）。对于化学预防评价，确定了生物转化系统的选定I相和II相酶、总抗氧化活性和动物的维生素E状态。使用合成的白藜芦醇缀合物标准品，通过高效液相色谱-二极管阵列检测（HPLC-DAD）鉴定和定量白藜芦醇及其代谢产物在粪便、尿液、血浆、肝脏和肾脏中的水平。喂食不同剂量的白藜芦醇对不同的化学预防参数没有影响，除了总的抗氧化活性，在喂食50 mg白藜芦醇/kg体重和每天后，血浆中的抗氧化活性升高了19%。通过HPLC分析检测反式-白藜芦醇-3-硫酸酯、反式-白藜芦醇-4-硫酸酯、反式-白藜芦醇-3，5-二硫酸酯、反式-白藜芦醇-3，4 '-二硫酸酯、反式-白藜芦醇-3，4'，5 -三硫酸酯、反式-白藜芦醇-3-0-β-D-葡糖苷酸和白藜芦醇糖苷配基的形成，这取决于生物材料。总白藜芦醇回收率在尿和粪便中喂养50毫克白藜芦醇每公斤体重和天分别为15%和13%。对于大鼠喂食300 mg白藜芦醇/kg体重和日恢复率分别为54%和17%。这是第一个研究与相关的白藜芦醇共轭物的合成标准进行。缺乏对化学预防参数的影响可能是由于各种白藜芦醇缀合物的形成降低了其在大鼠中的生物利用度。
The purpose of this study was to investigate the implications of selected chemopreventive parameters and metabolic conversion of resveratrol in vivo. In two 8-week long feeding experiments with rats, a low-resveratrol diet containing 50 mg resveratrol per kg body weight (bw) and day and a high-resveratrol diet with 300 mg per kg bw and day were administered. For chemopreventive evaluation selected phase I and phase II enzymes of the biotransformation system, the total antioxidant activity, and the vitamin E status of the animals were determined. The level of resveratrol and its metabolites in the feces, urine, plasma, liver, and kidneys was identified and quantitated by high-performance liquid chromatography-diode array detection (HPLC-DAD) using synthesized resveratrol conjugate standards. Feeding of different dosages of resveratrol revealed no effect on the different chemopreventive parameters, except for the total antioxidant activity, which was elevated in plasma by 19% after feeding 50 mg resveratrol per kg bw and day. The formation of trans-resveratrol-3-sulfate, trans-resveratrol-4-sulfate, trans-resveratrol-3,5-disulfate, trans-resveratrol-3,4'-disulfate, trans-resveratrol-3,4', 5 -trisulfate, trans-resveratrol-3-O-beta-D-glucuronide, and resveratrol aglycone was detected by HPLC analysis, depending on the biological material. Total resveratrol recovery in urine and feces of rats fed on 50 mg resveratrol per kg bw and day was 15% and 13%, respectively. For rats fed the higher dosage of 300 mg resveratrol per kg bw and day recovery was 54% and 17%, respectively. This is the first study performed with synthesized standards of relevant resveratrol conjugates. The lack of effect on the chemopreventive parameters is probably due to the formation of various resveratrol conjugates reducing its bioavailability in the rat.