Inflammation and resolution are associated with upregulation of fatty acid β-oxidation in Zymosan-induced peritonitis.
Inflammation and resolution are associated with upregulation of fatty acid β-oxidation in Zymosan-induced peritonitis.
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DOI：
10.1371/journal.pone.0066270
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Fukusaki E
中科院分区：
文献类型：
--
作者：
Fujieda Y;Manno A;Hayashi Y;Rhodes N;Guo L;Arita M;Bamba T;Fukusaki E
炎症是对有害刺激的一种基本防御性反应。然而，如果不消退，它可能会造成损害。为了避免这种损害，生物体开发了一种称为消炎的机制。在这里，我们应用非靶向代谢组学方法建立了一种无菌的、自我解决的急性炎症动物模型，即酵母多糖诱导的小鼠腹膜炎，以检测炎症和分解对代谢组谱的影响。在给予酵母多糖后，无论是在腹膜冲洗液(PWF)中还是在血浆中，都观察到了显著的和时间相关的代谢物变化。这些代谢变化与炎性趋化因子或细胞因子的产生有很好的相关性。在PWF中，在酵母多糖处理的小鼠中，可以检测到的大多数代谢物增加，这表明炎症、氧化应激和能量需求增加。在血浆中，给予酵母多糖后，中枢代谢途径(糖酵解和TCA循环)中的大多数代谢物显著下调。注射酵母多糖的动物血浆和肺组织中酮体3-羟基丁酸(3-Hb)浓度升高，提示脂肪酸β氧化上调。3-Hb水平在渗入腹膜的细胞中升高，这些渗入的细胞可能至少部分参与了腹膜腔内3-Hb的产生。
Inflammation is a fundamental defensive response to harmful stimuli. However, it can cause damage if it does not subside. To avoid such damage, organisms have developed a mechanism called resolution of inflammation. Here we applied an untargeted metabolomics approach to a sterile and self-resolving animal model of acute inflammation, namely zymosan-induced peritonitis in mice, to examine the effect of inflammation and resolution on the metabolomic profiles. Significant and time-dependent changes in metabolite profiles after zymosan administration were observed in both peritoneal wash fluid (PWF) and plasma. These metabolomic changes correlated well with inflammatory chemokine or cytokine production. In PWF, most of metabolites that could detected increased in zymosan-treated mice, which is suggestive of inflammation, oxidative stress and increased energy demands. In plasma, most metabolites in the central metabolic pathway (glycolysis and TCA cycle) were significantly downregulated after zymosan administration. The concentration of the ketone body 3-hydroxybutyric acid (3-HB) in plasma and PWF increased in zymosan-injected animals indicating upregulation of fatty acid β-oxidation. Increased 3-HB level was observed in the cells that infiltrated into the peritoneal cavity and these infiltrated cells might contribute, at least in part, to the production of 3-HB in the peritoneal cavity.
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DOI：
10.1074/jbc.m112.340612
发表时间：
2012-03-23
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Isobe Y;Arita M;Matsueda S;Iwamoto R;Fujihara T;Nakanishi H;Taguchi R;Masuda K;Sasaki K;Urabe D;Inoue M;Arai H
通讯作者：
Arai H
影响因子：
7.4
作者：
Lommen, Arjen
通讯作者：
Lommen, Arjen
DOI：
10.1621/nrs.08002
发表时间：
2010-04-16
期刊：
Nuclear receptor signaling
影响因子：
--
作者：
Pyper, Sean R;Viswakarma, Navin;Reddy, Janardan K
通讯作者：
Reddy, Janardan K
影响因子：
4.8
作者：
Liu, Tie Fu;Vachharajani, Vidula T.;McCall, Charles E.
通讯作者：
McCall, Charles E.
影响因子：
5.5
作者：
Gijón, MA;Leslie, CC
通讯作者：
Leslie, CC