Flutamide induces ultrastructural changes in spermatids and the ectoplasmic specialization between the Sertoli cell and spermatids in mouse testes
Flutamide induces ultrastructural changes in spermatids and the ectoplasmic specialization between the Sertoli cell and spermatids in mouse testes
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DOI：
10.1016/j.reprotox.2004.02.011
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发表时间：
2004-06-01
影响因子：
3.3
通讯作者：
Mori, C
中科院分区：
文献类型：
--
作者：
Anahara, R;Toyama, Y;Mori, C
Fluoride（Flu）是一种抗雄激素化合物，可破坏男性雄激素依赖组织的发育。本研究旨在通过电镜观察流感病毒对ICR小鼠睾丸的影响。新生小鼠在第2、4、6、8、10和12天（第1天：出生当天）皮下注射0.00012、0.0012、0.012、0.12、1.2、12或120 μ g Flu/g体重/注射。此外，成年小鼠连续5天注射0.0012、0.012、0.12或1.2 μ g Flu/g体重/天。处理睾丸进行电子显微镜检查。在新生儿治疗中，精子细胞的顶体和/或细胞核变形。此外，支持细胞和精子细胞之间的外质特化部分或全部缺失。生精周期的各个阶段在新生儿治疗中也被打乱。新生儿和成人治疗的影响之间没有超微结构差异，但是，在成人治疗中未观察到阶段紊乱。经腹腔给药的小鼠中异常精子细胞的百分比高于成年后给药的小鼠。由于在用β-雌二醇-3-苯甲酸酯（E2 B）处理后报告了类似的观察结果，因此Flu的存在可能在小鼠睾丸中诱导“异种雌激素环境”。(C)2004年爱思唯尔公司All rights reserved.
Flutamide (Flu) is an anti-androgenic compound that disrupts development of male androgen-dependent tissues. The purpose of the present study was to investigate the effects of Flu on ICR mouse testes by electron microscopic observation. Newborn mice were subcutaneously injected with 0.00012, 0.0012, 0.012, 0.12, 1.2, 12 or 120 mug Flu/g body weight/shot on Days 2, 4, 6, 8, 10 and 12 (Day 1: day of birth). In addition, adult mice were injected with 0.0012, 0.012, 0.12 or 1.2 mug Flu/g body weight/day for 5 sequential days. Testes were processed for electron microscopy. In neonatal treatments, acrosomes and/or nuclei of the spermatids were deformed. In addition, the ectoplasmic specialization between the Sertoli cell and spermatids was partially or completely deleted. Stages of the seminiferous cycle were also disarranged in the neonatal treatments. There were no ultrastructural differences between the effects of neonatal and adult treatments, however, stage disarrangement was not observed in adult treatments. The percentages of abnormal spermatids were higher in neonatally treated mice than in mice treated as adults. Since similar observations were reported after treatment with beta-estradiol-3-benzoate (E2B), the presence of Flu may induce a "xenoestrogenic environment" in mouse testes. (C) 2004 Elsevier Inc. All rights reserved.