Receptor-mediated regulation of superoxide production in human neutrophils stimulated by phorbol myristate acetate.
Receptor-mediated regulation of superoxide production in human neutrophils stimulated by phorbol myristate acetate.
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佛波醇肉豆蔻酸酯乙酸酯刺激的人中性粒细胞中超氧化物产生的受体介导调节。
DOI：
10.1172/jci110378
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Cohen,L
中科院分区：
文献类型：
--
作者：
Lehrer,RI;Cohen,L
人类中性粒细胞含有肉豆蔻酸酯（PMA）受体，PMA是一种复杂的脂质，可诱导它们产生超氧化物（O(2)）。PMA与这些受体结合表现出特异性、可逆性和高亲和力。受体的表观KD约为0.29 nM，每个中性粒细胞存在多个拷贝（约2.1 +/- 0.6 x 10(5)）。我们发现中性粒细胞呼吸爆发的时间和幅度是独立设定的。O(2)的产生发生在滞后之后，滞后与添加PMA的初始浓度成反比。O(2)产生的程度（速率）与PMA对受体的占有比例成正比。双重调节控制，如我们注意到的中性粒细胞受PMA刺激时的双重调节控制，可以在面对短暂或低强度刺激时提供代谢稳定性，而不会损害对更大干扰的快速和有力反应。
Human neutrophils contain receptors for phorbol myristate acetate (PMA), a complex lipid that induces them to generate superoxide (O (2)). Binding of PMA to these receptors displays specificity, reversibility, and high affinity. The receptor's apparent KD was approximately 0.29 nM and multiple copies (approximately 2.1 +/- 0.6 x 10(5)) were present per neutrophil. We found that the timing and magnitude of the neutrophil's respiratory burst were set independently. The onset of O (2) production occurred after a lag that was inversely proportional to the initial concentration of added PMA. The extent (rate) of O (2) production was directly proportional to the fractional occupancy of the receptor by PMA. Dual regulatory controls, such as those we noted when neutrophils were stimulated by PMA, could afford metabolic stability in the face of transient or low intensity stimuli without compromising quick and powerful responses to larger disturbances.