Improvement of the immunity of pig to Hog cholera vaccine by recombinant plasmid with porcine interleukin-6 gene and CpG motifs
Improvement of the immunity of pig to Hog cholera vaccine by recombinant plasmid with porcine interleukin-6 gene and CpG motifs
复制标题
猪白细胞介素6基因和CpG基序重组质粒提高猪对猪霍乱疫苗的免疫力
DOI：
10.1016/j.vaccine.2011.03.036
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发表时间：
2011-05-17
期刊：
影响因子：
5.5
通讯作者：
Gao, Rong
中科院分区：
文献类型：
--
作者：
Li, Dong;Chen, Jian-Lin;Gao, Rong
为了观察重组猪白细胞介素6基因（IL-6）和CpG基序对猪疫苗免疫应答的剂量效应，采用离子交联法制备了含有IL-6基因和11个CpG基序的免疫刺激序列的壳聚糖纳米粒（CNP），并将其包装于重组真核表达质粒VPIL-6C中。然后分别以0.5、1.0和1.5mg/头的剂量对30日龄仔猪进行肌肉注射。同时，将仔猪随机分为A1、A2、A3组，分别注射猪瘟弱毒疫苗。免疫后每周采血检测免疫球蛋白、特异性抗体、白细胞介素、IFN-γ和免疫细胞的变化。结果发现，与注射VR 1020-CNP的对照仔猪相比，在免疫后14至70天，处理组、对照组和处理组的血清中IgG、伊加和IgM、特异性抗体、IL-2、IL-6和IFN-γ的含量显著升高VPIL 6C组仔猪血液中T-H、T-C和CD 3（+）阳性T细胞数显著升高（P <0. 05）。接种后14 ~ 56 d，A1组上述免疫指标均不同程度地低于A2和A3组（P > 0.05）。淋巴细胞计数也明显升高（P < 0.05）。说明CNP包埋的VPIL 6C是一种新型的有效佐剂，能增强猪对猪瘟的体液免疫和细胞免疫，具有增强猪对传染病抵抗力的潜力。(C)2011爱思唯尔有限公司保留所有权利。
In order to observe the dosage-effect of recombinant pig interleukin-6 gene and CpG motifs on the immune responses of swine to vaccine, a novel recombinant eukaryotic VPIL6C plasmid was packed with chitosan nanoparticles (CNP) prepared by ionic cross linkage, which contains pig interleukin-6 gene and immunostimulatory sequence consisted of 11 CpG motifs. CNP-VRIL6C was then utilized to inoculate 30-day-old piglets intramuscularly at the dosage of 0.5, 1.0 and 1.5 mg/per capita, respectively. Meanwhile, the piglets were injected with attenuated classical Hog cholera vaccine and designated as A1, A2 and A3 group. The blood was weekly collected from the piglets after vaccination to detect the changes of immunoglobulins, specific antibody, interleukins, IFN-gamma and immune cells. The results were found that compared to those of the control piglets injected with VR1020-CNP, the content of IgG, IgA and IgM, specific antibodies, IL-2, IL-6 and IFN-gamma significantly increased in the sera from the treated three groups from 14 to 70 days after vaccination (P < 0.05); the number of T-H, T-C and CD3(+) positive T cells raised obviously in the blood of VPIL6C treated piglets (P < 0.05). Also the above immune indexes of A1 group were significantly lower to different extent in comparison with those of A2 and A3 group from 14 to 56 days post inoculation (P > 0.05). Moreover, the lymphocytes also remarkably elevated in the treated groups (P < 0.05). These indicate that VPIL6C entrapped with CNP is a novel effective adjuvant to boost the humoral and cellular immunity of pig to Hog cholera, implying it's potentiality to enhance the resistance of pig against infectious diseases. (C) 2011 Elsevier Ltd. All rights reserved.