FURTHER-STUDIES ON ACTIVATION OF PROCOLLAGENASE, LATENT PRECURSOR OF BONE COLLAGENASE - EFFECTS OF LYSOSOMAL CATHEPSIN-B, PLASMIN AND KALLIKREIN, AND SPONTANEOUS ACTIVATION
FURTHER-STUDIES ON ACTIVATION OF PROCOLLAGENASE, LATENT PRECURSOR OF BONE COLLAGENASE - EFFECTS OF LYSOSOMAL CATHEPSIN-B, PLASMIN AND KALLIKREIN, AND SPONTANEOUS ACTIVATION
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DOI：
10.1042/bj1660021
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发表时间：
1977-01-01
影响因子：
4.1
通讯作者：
VAES, G
中科院分区：
文献类型：
--
作者：
EECKHOUT, Y;VAES, G
组织蛋白酶B（[EC 2.4.22.1]，一种组织（溶酶体）蛋白酶）和2种体液蛋白酶（纤溶酶[EC 3.4.21.7]和激肽释放酶[EC 3.4.21.8]）可激活培养物中小鼠骨外植体释放的潜伏胶原酶[EC3.4.24.3]（胶原原酶）。其他溶酶体蛋白酶（羧肽酶B、组织蛋白酶C和D）和凝血酶不能激活前胶原酶。在4 ℃下用3 M-NaSCN透析培养液。对于某些培养液，延长25 ℃预孵育时间。C也引起前胶原酶的激活。在所有这些情况下，前胶原酶的活化涉及至少2个连续步骤：存在于培养液中的内源性潜伏活化剂的活化和前胶原酶本身的活化。开发了内源性激活剂的测定方法。人血清、牛血清白蛋白、酪蛋白和半胱氨酸在不影响胶原酶活性的浓度下抑制内源性激活剂。N-乙基马来酰亚胺和4-羟基汞苯甲酸刺激内源性激活剂，但碘乙酸没有影响。组织蛋白酶B、激肽释放酶和纤溶酶可能在潜在胶原酶的生理激活中起作用，从而启动胶原在体内的降解。无论原胶原酶（酶原或酶抑制剂复合物）的分子性质如何，这都可能发生。
Cathepsin B, [EC 2.4.22.1] a tissue (lysosomal) proteinase, and 2 humoral proteinases, plasmin [EC 3.4.21.7] and kallikrein, [EC 3.4.21.8] activate the latent collagenase [EC 3.4.24.3] (procollagenase) which is released by mouse bone explants in culture. Other lysosomal proteinases (carboxypeptidase B, cathepsin C and D) and thrombin did not activate the procollagenase. Dialysis of the culture fluids against 3 M-NaSCN at 4.degree. C and, for some culture fluids, prolonged preincubation at 25.degree. C also caused the activation of procollagenase. In all these cases, activation of procollagenase involved at least 2 successive steps: the activation of an endogenous latent activator present in the culture fluids and the activation of procollagenase itself. An assay method was developed for the endogenous activator. Human serum, bovine serum albumin, casein and cysteine inhibited the endogenous activator at concentrations that did not influence the collagenase activity. N-Ethylmaleimide and 4-hydroxymercuribenzoate stimulated the endogenous activator, but iodoacetate had no effect. It is proposed that cathepsin B, kallikrein and plasmin may play a role in the physiological activation of latent collagenase and thus initiate degradation of collagen in vivo. This may occur whatever the molecular nature of procollagenase (zymogen or enzyme-inhibitor complex) might be.