Anthocyanin Production in Suspension Cultures of High-producing Cells of Euphorbia millii
Anthocyanin Production in Suspension Cultures of High-producing Cells of Euphorbia millii
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大戟高产细胞悬浮培养花青素的产生
DOI：
10.1271/bbb1961.53.417
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Yasuyuki Yamada
中科院分区：
文献类型：
--
作者：
Yoshikazu Yamamoto;Y. Kinoshita;Satomi Watanabe;Yasuyuki Yamada
我们研究了通过连续的细胞聚集体选择获得的千金子细胞株的悬浮培养中花青素苷生产的最佳条件。改良甘氏液含蔗糖5 ~ 7%（w/v），氮源30 mm，4-D 10 6 m2，BA 10 8 m3，Fe 2+浓度低，配比1：16，可显著提高花色素苷产量。2，4-D对花青苷产量的促进作用上级优于NAA。在建立的花色苷生产培养基中，E. 100万个细胞每天每升产生32 mg花青素。
We investigated the optimal conditions for anthocyanin production in suspension cultures of a Euphorbia millii cell strain obtained by successive cell-aggregate selections. A modified Gamborg’s solution containing 5 to 7% sucrose (w/v), a 1:16 ratio of , 30 mm of nitrogen source, 10 6 m2,4-D, 10 8 m BA, and low concentrations of Fe2+ and greatly increased anthocyanin production. 2,4-D was superior to NAA in increasing anthocyanin production. In the anthocyanin production medium we established, E. millii cells produced 32 mg anthocyanin per liter per day.