Activation of caspase-3 in axotomized rat retinal ganglion cells in vivo
Activation of caspase-3 in axotomized rat retinal ganglion cells in vivo
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DOI：
10.1016/s0014-5793(99)00747-4
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发表时间：
1999-06-25
期刊：
影响因子：
3.5
通讯作者：
Bähr, M
中科院分区：
文献类型：
--
作者：
Kermer, P;Klöcker, N;Bähr, M
最近，我们已经表明，抑制半胱天冬酶-3样半胱天冬酶是最有效的治疗策略，以保护成年大鼠视网膜神经节细胞继发性死亡后，视神经横断。在本研究中，我们本地化的活性caspase-3在轴突切断的视网膜神经节细胞在体内，并证明了共同定位的活性p20片段和TUNEL染色在这些细胞中的一些。与此相一致，我们检测到损伤后视网膜组织中caspase-3蛋白的切割和活性增强，而caspase-3 mRNA表达保持不变。这些数据表明，半胱天冬酶-3作为一个重要的介质的继发性视网膜神经节细胞死亡后轴突在体内。(C)1999年欧洲生物化学学会联合会。
Recently, we have shown that inhibition of caspase-3-like caspases is the most effective treatment strategy to protect adult rat retinal ganglion cells from secondary death following optic nerve transection. In the present study, we localized active caspase-3 in axotomized retinal ganglion cells in vivo and demonstrated a co-localization of the active p20 fragment and TUNEL-staining in some of these cells. In line with this, we detected an enhanced cleavage and activity of caspase-3 protein in retinal tissue after lesion, while caspase-3 mRNA expression remained unchanged. These data suggest caspase-3 as an important mediator of secondary retinal ganglion cell death following axotomy in vivo. (C) 1999 Federation of European Biochemical Societies.