Effect of fatty acid chain length and saturation on the gastrointestinal handling and metabolic disposal of dietary fatty acids in women
Effect of fatty acid chain length and saturation on the gastrointestinal handling and metabolic disposal of dietary fatty acids in women
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DOI：
10.1017/s0007114599000872
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发表时间：
1999-01-01
影响因子：
3.6
通讯作者：
Wootton, SA
中科院分区：
文献类型：
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作者：
Jones, AE;Stolinski, M;Wootton, SA
通过测定粪便中[1-C-13]标记和呼气时排出的[1-C-13]标记为(CO2)-C-13的量和性质，研究了[1-C-13]棕榈酸、[1-C-13]硬脂酸和[1-C-13]油酸在脂肪-酪蛋白-葡萄糖-蔗糖乳剂中的胃肠道处理和代谢处置。C-13标记在粪便中排泄最多的是硬脂酸试验(给药量的9.2%)，而在棕榈酸(给药剂量的1.2%)或油酸(给药剂量的1.9%)试验的粪便中观察到的标记相对较少。在棕榈酸和油酸试验中，粪便中的所有标记都被确认为以给药的形式存在(即[C-13]棕榈酸试验中的棕榈酸和油酸试验中的[C-13]油酸)。相比之下，硬脂酸试验中粪便中只有87%的标记被鉴定为[C-13]硬脂酸，其余的被鉴定为[C-13]棕榈酸，这可能反映了[1-C-13]硬脂酸在胃肠道内的链缩短。在研究期间的最初9小时内，在呼气中C-13标记恢复的时间过程中观察到了微小的，但有统计学意义的差异(油酸=棕榈酸和硬脂酸)。然而，当在24小时的研究期间计算时，在所有三个试验中标记为(CO2)-C-13的回收率相似(约为吸收剂量的25%)。这些结果支持这样的观点，即即使在乳剂中呈现这些脂肪酸，链的长度和不饱和度也可能影响这些脂肪酸的胃肠道处理和即时代谢处置。
The gastrointestinal handling and metabolic disposal of [1-C-13]palmitic acid, [1-C-13] stearic acid and [1-C-13]oleic acid administered within a lipid-casein-glucose-sucrose emulsion were examined in normal healthy women by determining both the amount and nature of the C-13 label in stool and label excreted on breath as (CO2)-C-13. The greatest excretion of C-13 label in stool was in the stearic acid trial (9.2 % of administered dose) whilst comparatively little label was observed in stool in either the palmitic acid (1.2 % of administered dose) or oleic acid (1.9 % of administered dose) trials. In both the palmitic acid and oleic acid trials, all of the label in stool was identified as being present in the form in which it was administered (i.e. [C-13]palmitic acid in the palmitic acid trial and [C-13]oleic acid in the oleic acid trial). In contrast, only 87 % of the label in the stool in the stearic acid trial was identified as [C-13]stearic acid, the remainder was identified as [C-13]palmitic acid which may reflect chain shortening of [1-C-13]stearic acid within the gastrointestinal tract. Small, but statistically significant, differences were observed in the time course of recovery of C-13 label on breath over the initial 9 h of the study period (oleic acid = palmitic acid > stearic acid). However, when calculated over the 24 h study period, the recovery of the label as (CO2)-C-13 was similar in all three trials (approximately 25 % of absorbed dose). These results support the view that chain length and degree of unsaturation may influence the gastrointestinal handling and immediate metabolic disposal of these fatty acids even when presented within an emulsion.