DNA sequence duplications trigger gene inactivation in Neurospora crassa.
DNA sequence duplications trigger gene inactivation in Neurospora crassa.
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DNA 序列重复会触发粗糙脉孢菌中的基因失活。
DOI：
10.1073/pnas.85.18.6870
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发表时间：
1988
影响因子：
11.1
通讯作者：
Garrett,PW
中科院分区：
文献类型：
--
作者：
Selker,EU;Garrett,PW
转化序列在脉孢菌的营养细胞中忠实地复制，但通常在受精和核融合之间的时期以高频率进行序列改变和甲基化。以前的工作表明，这些激进的变化，简称RIP，和引入的DNA导致的序列重复的存在之间的相关性。对RIP过程的各种可能原因进行了调查。将含有脉孢菌am基因的DNA片段的单个拷贝引入具有该DNA缺失的菌株中不会导致RIP，而引入相同片段的两个或更多个拷贝则会导致RIP。使用在非连锁染色体位置具有单拷贝am的菌株的常规杂交来构建针对am复制的菌株。在该菌株的杂交中，重复基因的两个拷贝都经历RIP过程。我们的结论是，序列重复，本身，触发RIP。
Transforming sequences are faithfully replicated in vegetative cells of Neurospora but are typically subject at high frequency to sequence alterations and methylation in the period between fertilization and nuclear fusion. Previous work showed a correlation between the occurrence of these radical changes, referred to by the acronym RIP, and the presence of sequence duplications resulting from the introduced DNA. Various possible causes for the RIP process were investigated. Introduction of a single copy of a DNA fragment containing the Neurospora am gene into a strain having a deletion of this DNA did not lead to RIP, whereas introduction of two or more copies of the same fragment did. A conventional cross of strains having single copies of am at unlinked chromosomal locations was used to build a strain duplicated for am. Both copies of the duplicated gene were subject to the RIP process in crosses of this strain. We conclude that sequence duplications, per se, trigger RIP.