INCORPORATION OF PLASTICIZER INTO RED-CELLS DURING STORAGE
INCORPORATION OF PLASTICIZER INTO RED-CELLS DURING STORAGE
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DOI：
10.1046/j.1537-2995.1984.24685066808.x
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发表时间：
1984-01-01
期刊：
影响因子：
2.9
通讯作者：
TACKABERRY, ES
中科院分区：
文献类型：
--
作者：
ROCK, G;TOCCHI, M;TACKABERRY, ES
柔性塑料血袋的发展使得有效的血液成分生产和治疗成为可能。然而，增塑剂邻苯二甲酸二（2-乙基己基）酯（DEHP）对人体的毒性仍不清楚，已知它会从塑料中渗入储存的血液中。尽管有不使用 DEHP 的塑料制成的袋子，但红细胞的收集和储存仍然在 DEHP 塑料包装中进行，事实上，最近使用新的抗凝剂-防腐剂解决方案将红细胞的储存期限延长至 49 天。研究了 DEHP 与储存的红细胞之间的关系。 28% 的可用 14C-DEHP 立即结合到红细胞的膜和细胞质部分中的位点，并且 14C-DEHP 的总量和分布在 7 天内没有显着变化。当红细胞浓缩物在含有或不含 DEHP 的情况下储存时，使用不含 DEHP 的塑料（聚烯烃）袋或玻璃，发现在不存在 DEHP 的情况下，红细胞的渗透稳定性明显降低。在含有 DEHP 的塑料包装中储存 35 天后，血浆游离血红蛋白水平为 90.3 毫克/分升，在聚烯烃袋中储存为 181.7 毫克/分升。必须权衡储存在含有 DEHP 的塑料中的红细胞的体外稳定性得到改善的优点与患者接触 DEHP 的潜在危害。
The development of flexible plastic blood bags has permitted effective blood component production and therapy. However, the plasticizer di(2-ethylhexyl) phthalate (DEHP), whose toxicity in humans is still undefined, is known to leach from the plastic into stored blood. Despite the availability of bags made of plastics not using DEHP, the collection and storage of red cells is still done in DEHP plasticized packs, and in fact the storage life for red cells was recently increased up to 49 days using new anticoagulant-preservative solutions. The relationship between DEHP and stored red cells was examined. Twenty-eight percent of available 14C-DEHP binds immediately to sites in both the membrane and cytosol fractions of the red cells, and the total amount and distribution of 14C-DEHP does not change significantly over 7 days. When red cell concentrates were stored with or without DEHP, using either plastic (polyolefin) bags not containing DEHP or glass, definite reduction in the osmotic stability of the red cells was found in the absence of DEHP. Plasma-free Hb levels were 90.3 mg per dl after 35 days of storage in plastic packs containing DEHP and 181.7 mg per dl in the polyolefin bags. The advantages of improved in vitro stability of red cells stored in plastics containing DEHP must be weighed against the potential hazards of patient exposure to DEHP.