Quantitative analysis of the seasonal and tissue-specific expression of Cry1Ab in transgenic maize Mon810
Quantitative analysis of the seasonal and tissue-specific expression of Cry1Ab in transgenic maize Mon810
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DOI：
10.1007/bf03356208
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发表时间：
2007-04-01
影响因子：
2
通讯作者：
Jehle, J. A.
中科院分区：
文献类型：
--
作者：
Nguyen, H. T.;Jehle, J. A.
在德国波恩和哈勒附近的两个田间试验中，测定了转基因玉米(MON810，品种‘Novelis’)中Cry1Ab蛋白的组织特异性表达和季节丰度。用双抗血清-酶联免疫吸附试验(DAS-EL ISA)对1085份样品进行分析。对2001、2002和2003年采集的4个不同生育期(BBCH19、BBCH30、BBCH61和BBCH83)的植物组织(根、茎、上部叶、下部叶、花药、花粉和籽粒)的Cry1Ab含量进行了测定。MON810在BBCH83的叶片中Cry1Ab含量最高(5.5-6.4mgg(-1)FW)，而在花粉中含量最低(1-97ngg(-1)FW)。收获后9个月田间残根Cry1Ab含量为15~17ngg(-1)FW。这说明残根中的Cry1Ab浓度降低到鲜根的百分之一左右。对Cry1Ab表达的监测表明，不同植物个体之间的Cry1Ab含量差异很大。
The tissue-specific expression and seasonal abundance of Cry1Ab protein were determined in transgenic maize plants (Mon810, variety 'Novelis') from two field trials located near Bonn and Halle, Germany. A total of 1085 samples were analysed by using Double Antiserum-Enzyme Linked Immunosorbent Assay (DAS-ELISA). The Cry1Ab contents of various plant tissues (root, stem, upper leaf, lower leaf, anther, pollen and kernel) were determined at four different growth stages (BBCH19, BBCH30, BBCH61 and BBCH83) collected in 2001, 2002 and 2003. Mon810 showed the highest Cry1Ab contents in the leaves (5.5 - 6.4 mu g g(-1) fresh weight [fw]) at BBCH83, whereas the lowest Cry1Ab contents were detected in the pollen (1 - 97 ng g(-1) fw). Cry1Ab content of residual root stocks collected in the field nine months after harvest was 15 - 17 ng g(-1) fw. This demonstrated that the Cry1Ab concentration in residual root stocks was reduced to about one-hundredth of the fresh roots. The monitoring of Cry1Ab expression showed that the Cry1Ab contents varied strongly between different plant individuals.