Enzyme patterns in mitochondria of eggs, liver, and skeletal muscle during larval development of Xenopus.
Enzyme patterns in mitochondria of eggs, liver, and skeletal muscle during larval development of Xenopus.
复制标题
非洲爪蟾幼虫发育过程中卵、肝脏和骨骼肌线粒体中的酶模式。
DOI：
10.1016/0012-1606(74)90145-6
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发表时间：
1974
影响因子：
2.7
通讯作者：
R. Weber
中科院分区：
文献类型：
--
作者：
A. Kistler;R. Weber
本研究的目的是比较选择酶的分布在线粒体的卵和分化的幼虫组织（肝脏，尾肌）的爪蟾。为此，研究了五种基质酶的比活性，（谷氨酸脱氢酶、谷氨酸草酰乙酸转氨酶、谷氨酸丙酮酸转氨酶、3-羟酰基-CoA脱氢酶和苹果酸脱氢酶）和三种膜结合酶（甘油磷酸脱氢酶，3-羟基丁酸脱氢酶，琥珀酸脱氢酶）包括细胞色素aa 3含量测定线粒体分离的差速离心和纯化的离心在蔗糖密度梯度。酶含量的最大差异被发现之间的线粒体的卵和那些分化的组织。在幼虫发育过程中，肝脏和尾肌的线粒体酶谱变化不大。基质酶变化最大，而膜结合呼吸酶SDH和细胞色素aa 3变化不大，这也与不溶性线粒体蛋白质含量呈显著相关性。通过将线粒体酶活性与细胞色素aa 3含量联系起来，获得了组织特异性活性模式。根据变异程度，可以区分具有“恒定”和“比活性比例”的酶，每组包括膜结合酶和基质酶。这表明，酶的含量可能独立于线粒体中的假定结构实体而变化。
The purpose of this investigation was to compare the distribution of selected enzymes in mitochondria of eggs and in differentiated larval tissues (liver, tail muscle) ofXenopus. To this end the specific activities of five matrix enzymes (glutamate dehydrogenase, glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, 3-hydroxyacyl-CoA dehydrogenase, and malate dehydrogenase) and three membrane-bound enzymes (glycerolphosphate dehydrogenase, 3-hydroxybutyrate dehydrogenase, succinate dehydrogenase) including the cytochromeaa3content were determined on mitochondria isolated by differential centrifugation and purified by centrifugation in a sucrose density gradient.The greatest differences in enzyme content were found between mitochondria of eggs and those of the differentiated tissues. During larval development there was only little change in the mitochondrial enzyme patterns of liver and tail muscle. The greatest variation was found in matrix enzymes, but only little change was noted in the membrane-bound respiratory enzyme SDH and cytochromeaa3which also showed a striking correlation with the amount of insoluble mitochondrial protein.By relating mitochondrial enzyme activities to cytochromeaa3content, tissue specific activity patterns were obtained. Depending upon the degree of variation, it was possible to differentiate enzymes with “constant” and “specific activity proportions,” each group comprising membrane-bound and matrix enzymes. This indicates that enzyme content may vary independently of its presumed structural entity in the mitochondria.