Host-mediated RNA interference targeting acuticular protein gene impaired fecundity inthe green peach aphid Myzus persicae
Host-mediated RNA interference targeting acuticular protein gene impaired fecundity inthe green peach aphid Myzus persicae
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DOI：
10.1002/ps.4900
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发表时间：
2018-09-01
影响因子：
4.1
通讯作者：
Bhattacharya, Ramcharan
中科院分区：
文献类型：
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作者：
Bhatia, Varnika;Bhattacharya, Ramcharan
背景技术桃蚜（Myzus persicae）是一种毁灭性的吸汁害虫，它损害全世界许多寄主植物并造成数十亿美元的农作物损失。通过口服小干扰 RNA (siRNA) 诱导 RNA 干扰 (RNAi) 已在蚜虫中得到证实。因此，宿主介导的对蚜虫重要结构基因特异的双链 RNA (dsRNA) 的传递被设想为开发针对该蚜虫物种的抗性的工具。 结果表皮蛋白 (CP) 感知季节性光周期并驱动蚜虫从无性繁殖到有性繁殖的转变。因此，CP基因表达的减弱可能会导致蚜虫的生殖方向不同，从而影响其繁殖力。桃桃中编码 CP 的基因已成为 RNAi 介导的敲低目标。开发了表达与 MyCP 基因同源的 dsRNA 的转基因拟南芥。 dsRNA 转基因产生由侵染转基因的蚜虫喂养的基因特异性 siRNA。逆转录定量聚合酶链反应（RT-qPCR）研究表明，在转基因植物上饲养的蚜虫中，CP 基因的转录物水平降低。 CP基因表达的减少导致转基因拟南芥植物上蚜虫繁殖力显着下降。结论增加遗传抗性是最大限度地减少有毒农用化学品的使用以保护植物的唯一可持续方法。宿主介导的重要昆虫基因的 RNAi 已被提议作为培养作物对害虫的抗性的潜在途径。这项研究证明了 MyCP dsRNA 在开发基于 RNAi 的桃蚜抗性方面的潜力。与其他转基因策略相比，RNAi 介导的耐药性预计会更持久。 (c) 2018年化学工业学会
BACKGROUNDThe green peach aphid (Myzus persicae) is a devastating sap-sucking insect pest that damages many host plants worldwide and causes billions of dollars of crop losses. Induction of RNA interference (RNAi) through oral feeding of small interfering RNA (siRNA) has been demonstrated in aphids. Therefore, host-mediated delivery of double-stranded RNA (dsRNA) specific to vital structural genes of aphids has been envisaged as a tool for the development of resistance against this aphid species.RESULTSCuticular protein (CP) senses seasonal photoperiodism and drives a shift from clonal to sexual generation in aphids. Thus, attenuation of CP gene expression is likely to result in a different reproductive orientation in aphids and thereby affect their fecundity. A gene encoding CP in M. persicae has been targeted for RNAi-mediated knockdown. Transgenic Arabidopsis expressing dsRNA homologous to the MyCP gene was developed. The dsRNA-transgenics produced gene-specific siRNAs fed by aphids infesting the transgenics. A reverse transcription-quantitative polymerase chain reaction (RT-qPCR) study revealed an attenuated level of transcripts of the CP gene in aphid nymphs reared on the transgenic plants. Decreased expression of the CP gene resulted in a noticeable decline in aphid fecundity on the transgenic Arabidopsis plants.CONCLUSIONIncreasing genetic resistance is the only sustainable way of minimizing the use of toxic agrochemicals to protect plants. Host-mediated RNAi of important insect genes has been proposed as a potential avenue for developing crop resistance against insect pests. This study demonstrated the potential of MyCP dsRNA in developing RNAi-based resistance to M. persicae. RNAi-mediated resistance is expected to be more durable compared with other transgenic strategies. (c) 2018 Society of Chemical Industry