TaOPR2 encodes a 12-oxo-phytodienoic acid reductase involved in the biosynthesis of jasmonic acid in wheat (Triticum aestivum L.).
TaOPR2 encodes a 12-oxo-phytodienoic acid reductase involved in the biosynthesis of jasmonic acid in wheat (Triticum aestivum L.).
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DOI：
10.1016/j.bbrc.2016.01.043
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发表时间：
2016-01
影响因子：
3.1
通讯作者：
Yukun Wang;Guoliang Yuan;Shao-hua Yuan;Wen-jing Duan;Peng Wang;Jian-fang Bai;Feng-ting Zhang;
中科院分区：
文献类型：
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作者：
Yukun Wang;Guoliang Yuan;Shao-hua Yuan;Wen-jing Duan;Peng Wang;Jian-fang Bai;Feng-ting Zhang;
12-氧代植物二烯酸还原酶（12-oxo-phytodienoic acid reductases，OPR）参与植物生长发育的各个过程，分为OPRⅠ和OPRⅡ两个亚类。在高等植物中，只有OPRⅡ亚类基因参与内源茉莉酸的生物合成。本研究从温敏核不育小麦品种BS 366中克隆了一个新的OPRⅡ亚类基因TaOPR 2（GeneBank登录号：KM 216389），该基因编码390个氨基酸。TaOPR 2基因定位于小麦7 B染色体上，定位于过氧化物酶体上。蛋白进化分析表明，TaOPR 2属于OPRⅡ亚类，与其他高等植物OPR蛋白具有高度的同源性。荧光定量PCR结果表明，TaOPR 2基因的表达受到阿坝、SA、GA 3、低温和高盐的抑制。而TaOPR 2的表达则受到伤害、干旱和茉莉酸甲酯（MeJA）的诱导。此外，TaOPR 2基因在小麦条锈菌和小麦反锈菌侵染后转录水平均有所提高。TaOPR 2具有NADPH依赖性氧化还原酶活性。此外，TaOPR 2的组成型表达可以挽救拟黑色素瘤受体3的雄性不育表型。这些结果表明TaOPR 2参与了小麦茉莉酸（JA）的生物合成。
The 12-oxo-phytodienoic acid reductases (OPRs) are involved in the various processes of growth and development in plants, and classified into the OPRⅠ and OPRⅡ subgroups. In higher plants, only OPRⅡ subgroup genes take part in the biosynthesis of endogenous jasmonic acid. In this study, we isolated a novel OPRⅡ subgroup gene namedTaOPR2(GeneBank accession: KM216389) from the thermo-sensitive genic male sterile (TGMS) wheat cultivar BS366.TaOPR2was predicted to encode a protein with 390 amino acids. The encoded protein contained the typical oxidored_FMN domain, the C-terminus peroxisomal-targeting signal peptide, and conserved FMN-binding sites.TaOPR2was mapped to wheat chromosome 7B and located on peroxisome. Protein evolution analysis revealed that TaOPR2 belongs to the OPRⅡ subgroup and shares a high degree of identity with other higher plant OPR proteins. The quantitative real-time PCR results indicated that the expression ofTaOPR2is inhibited by abscisic acid (ABA), salicylic acid (SA), gibberellic acid (GA3), low temperatures and high salinity. In contrast, the expression ofTaOPR2can be induced by wounding, drought and methyl jasmonate (MeJA). Furthermore, the transcription level ofTaOPR2increased after infection withPucciniastriiformisf.sp.triticiandPuccinia reconditef.sp.tritici. TaOPR2 has NADPH-dependent oxidoreductase activity. In addition, the constitutive expression ofTaOPR2can rescue the male sterility phenotype ofArabidopsismutantopr3. These results suggest thatTaOPR2is involved in the biosynthesis of jasmonic acid (JA) in wheat.