ABA-deficient (aba1) and ABA-insensitive (abi1-1, abi2-1) mutants of Arabidopsis have a wild-type stomatal response to humidity
ABA-deficient (aba1) and ABA-insensitive (abi1-1, abi2-1) mutants of Arabidopsis have a wild-type stomatal response to humidity
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DOI：
10.1046/j.1365-3040.2000.00551.x
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发表时间：
2000-04-01
影响因子：
7.3
通讯作者：
Lee, YRJ
中科院分区：
文献类型：
--
作者：
Assmann, SM;Snyder, JA;Lee, YRJ
在大多数植物物种中，叶表面大气湿度的降低会引发气孔导度的降低。虽然保卫细胞似乎对蒸腾速率的湿度诱导变化有反应，而非对相对湿度或水汽压差有反应，但这种反应的潜在细胞机制仍然未知。在当前这组实验中，使用了拟南芥脱落酸（ABA）缺陷型（aba1）和ABA不敏感型（abi1 - 1和abi2 - 1）突变体来检验以下假设：湿度信号是通过蒸腾流中输送到气孔复合体的ABA通量或浓度的变化来转导的。在气体交换实验中，aba1、abi1 - 1和abi2 - 1突变体植株的气孔导度对水汽压差的变化与野生型植株一样敏感。这些实验似乎排除了ABA或ABA结合物的浓度或通量作为保卫细胞对大气水势反应的介质的必然作用。这些结果与ABA在介导保卫细胞对土壤水势降低的反应中已确立的作用形成对比。
In most plant species, a decrease in atmospheric humidity at the leaf surface triggers a decrease in stomatal conductance. While guard cells appear to respond to humidity-induced changes in transpiration rate, as opposed to relative humidity or vapour pressure difference, the underlying cellular mechanisms for this response remain unknown. In the present set of experiments, abscisic acid (ABA)-deficient (aba1) and ABA-insensitive (abi1-1 and abi2-1) mutants of Arabidopsis thaliana were used to test the hypothesis that the humidity signal is transduced by changes in the flux or concentration of ABA delivered to the stomatal complex in the transpiration stream. In gas exchange experiments, stomatal conductance was as sensitive to changes in vapour pressure difference in aba1, abi1-1 and abi2-1 mutant plants as in wild-type plants. These experiments appear to rule out an obligate role for either the concentration or flux of ABA or ABA conjugates as mediators of the guard cell response to atmospheric water potential. The results stand in contrast to the well-established role of ABA in mediating guard cell responses to decreases in soil water potential.