Molecular cloning of two cDNAs from the head of the nurse honey bee (Apis mellifera L.) for coding related proteins of royal jelly
Molecular cloning of two cDNAs from the head of the nurse honey bee (Apis mellifera L.) for coding related proteins of royal jelly
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护士蜜蜂 (Apis mellifera L.) 头部的两个 cDNA 的分子克隆，用于编码蜂王浆相关蛋白
DOI：
10.1080/00218839.1994.11100857
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发表时间：
1994
影响因子：
1.9
通讯作者：
J. Šimúth
中科院分区：
文献类型：
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作者：
J. Klaudiny;J. Hanes;J. Kulifajová;S. Albert;J. Šimúth
从护士蜂头λUni-ZAP XR文库中鉴定出两个克隆，分别命名为pRJP57-1和pRJP57-2，它们编码主要王浆蛋白的相关多肽。插入片段的核苷酸序列同源性为70.2%。从pRJP57-1和pRJP57-2的开放阅读框推导出467和464个氨基酸残基的多肽的氨基酸序列，同源性为56.6%。没有观察到与迄今已知的蛋白质有显著同源性。这两种蛋白都含有N-糖基化位点、三种蛋白激酶的磷酸化位点和几乎相同的信号肽。Northern分析表明，总mRNA中含有较高比例的RJP57-1和RJP57-2mRNAs(分别为8%和2%)。
SUMMARYTwo cDNA clones designated pRJP57–1 and pRJP57–2 which code for related polypeptides of major royal jelly protein were identified from the λUni-ZAP XR library of nurse honey bee head cDNA. The nucleotide sequences of the cDNA inserts show a 70.2% identity. From the open reading frames of pRJP57–1 and pRJP57–2 the amino acid sequences for polypeptides of 467 and 464 amino acid residues were derived, showing an identity of 56.6%. No significant homology with hitherto known proteins was observed. Both proteins contain N-glycosylation sites, phosphorylation sites for three types of protein kinases and almost identical signal peptides. Northern analysis showed that total mRNA contains a high proportion of RJP57–1 and RJP57–2 mRNAs (8% and 2% respectively).
影响因子：
--
作者：
G. Pfeifer;A. Riggs
通讯作者：
G. Pfeifer;A. Riggs