Elucidation of the small RNA component of the transcriptome
Elucidation of the small RNA component of the transcriptome
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DOI：
10.1126/science.1114112
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发表时间：
2005-09-02
期刊：
影响因子：
56.9
通讯作者：
Green, PJ
中科院分区：
文献类型：
--
作者：
Lu, C;Tej, SS;Green, PJ
小RNA在大多数真核生物中起着重要的调节作用，但只有一小部分分子被鉴定出来。我们从模式植物拟南芥的幼苗和花序中测序了200多万个小RNA。已知和新的microRNAs(MiRNAs)是超过75000个序列的非冗余集合中最丰富的，而超过一半代表与重复序列、基因间隔区和基因匹配的较低丰度的小干扰RNA(SiRNAs)。很容易观察到单个或成簇的高度调控的小RNA。反义RNA或miRNA的靶标似乎并不优先与siRNA相关。许多以前被认为没有特征的基因组区域被发现是许多小RNA的位置。
Small RNAs play important regulatory roles in most eukaryotes, but only a small proportion of these molecules have been identified. We sequenced more than two million small RNAs from seedlings and the inflorescence of the model plant Arabidopsis thaliana. Known and new microRNAs (miRNAs) were among the most abundant of the nonredundant set of more than 75,000 sequences, whereas more than half represented lower abundance small interfering RNAs (siRNAs) that match repetitive sequences, intergenic regions, and genes. Individual or clusters of highly regulated small RNAs were readily observed. Targets of antisense RNA or miRNA did not appear to be preferentially associated with siRNAs. Many genomic regions previously considered featureless were found to be sites of numerous small RNAs.