High-Sensitivity Amperometric Biosensors Based on Ferrocene-Modified Linear Poly(ethylenimine)
High-Sensitivity Amperometric Biosensors Based on Ferrocene-Modified Linear Poly(ethylenimine)
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DOI：
10.1021/la9004938
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发表时间：
2009-07-07
期刊：
影响因子：
3.9
通讯作者：
Schmidtke, David W.
中科院分区：
文献类型：
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作者：
Merchant, Stephen A.;Tran, Tu O.;Schmidtke, David W.
通过将葡萄糖氧化酶（GOX）和辣根过氧化物酶（HRP）固定在二茂铁改性的线性聚（乙烯亚胺）交联膜中，构建了用于葡萄糖和过氧化氢的电流生物传感器。在 pH 7 时，葡萄糖传感器产生 1.2 mA/cm(2) 的极限催化电流密度。这些电流密度比其他二茂铁基氧化还原聚合物的电流密度高约 4 倍，并且与含有 GOX 的锇基氧化还原聚合物的最高报告值相当。由于这些薄膜的高灵敏度（73 nA/cm(2).mu M），可以检测微摩尔范围内的葡萄糖浓度。同样，传感器在饱和条件下构建为 HRP 产生的电流密度为 0.9 mA/cm(2)，灵敏度为 500 nA/cm(2).mu M。结果表明，Fc-LPEI 与多种酶有效通信的能力在测量可植入生物传感器中的低底物浓度和在酶生物燃料电池中产生高电流输出方面具有潜在的应用。
Amperometric biosensors for glucose and hydrogen peroxide have been built by immobilizing glucose oxidase (GOX) and horseradish peroxidase (HRP) in cross-linked films of ferrocene-modified linear poly(ethylenimine). At pH 7, the glucose sensors generated limiting catalytic current densities of 1.2 mA/cm(2). These current densities are approximately 4 times higher than those with other ferrocene-based redox polymers and are comparable to the highest reported values for osmium-based redox polymers with GOX. Because of the high sensitivity of these films (73 nA/cm(2).mu M), glucose concentrations in the micromolar range could be detected. Similarly, sensors were constructed with HRP-generated current densities of 0.9 mA/cm(2) under saturation conditions and sensitivities of 500 nA/cm(2).mu M. The results show that the ability of Fc-LPEI to effectively communicate with a variety of enzymes has potential applications in measuring low substrate concentrations in implantable biosensors and producing high current outputs in enzymatic biofuel cells.