An electrospun polycaprolactone–collagen membrane for the resurfacing of cartilage defects
An electrospun polycaprolactone–collagen membrane for the resurfacing of cartilage defects
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DOI：
10.1002/pi.2792
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发表时间：
2010-06
影响因子：
3.2
通讯作者：
S. T. Ho;A. K. Ekaputra;J. Hui;D. Hutmacher
中科院分区：
文献类型：
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作者：
S. T. Ho;A. K. Ekaputra;J. Hui;D. Hutmacher
聚己内酯（PCL）-胶原静电纺丝网被提出作为一种新的替代传统的骨膜移植自体软骨细胞植入。这是第一个已知的尝试，在设计一个软骨表面置换膜使用机械弹性PCL网的重均分子量为139 - 300，增强与生物活性胶原蛋白。对PCL-胶原10、20和40%电纺补片（科尔-10、科尔-20和科尔-40）进行了评价，发现仅在科尔-20和科尔-40中可实现表面胶原的保留。此外，科尔-20比科尔-40更硬和更强，并且其满足软骨植入部位的机械要求。当用间充质干细胞（MSC）接种时，细胞粘附在科尔-20网的表面上，并且它们在28天的时间内保持活力;然而，它们不能渗透通过致密的网。在软骨形成环境中也注意到细胞相容性，因为MSC分化成具有Sox 9、聚集蛋白聚糖和胶原II表达的软骨细胞。更重要的是，补片没有诱导细胞的肥大反应。目前的研究结果支持使用科尔-20作为软骨补片，并预计未来的植入研究。版权所有© 2010化学工业协会
A polycaprolactone (PCL)–collagen electrospun mesh is proposed as a novel alternative to the conventional periosteal graft in autologous chondrocyte implantation. This is the first known attempt in designing a cartilage resurfacing membrane using a mechanically resilient PCL mesh with a weight-average molecular weight of 139 300 that is enhanced with bioactive collagen. PCL–collagen 10, 20 and 40% electrospun meshes (Coll-10, Coll-20 and Coll-40) were evaluated and it was discovered that the retention of surface collagen could only be achieved in Coll-20 and Coll-40. Furthermore Coll-20 was stiffer and stronger than Coll-40 and it satisfied the mechanical demands at the cartilage implant site. When seeded with mesenchymal stem cells (MSCs), the cells adhered on the surface of the Coll-20 mesh and they remained viable over a period of 28 days; however, they were unable to infiltrate through the dense meshwork. Cell compatibility was also noted in the chondrogenic environment as the MSCs differentiated into chondrocytes with the expression of Sox9, aggrecan and collagen II. More importantly, the mesh did not induce a hypertrophic response from the cells. The current findings support the use of Coll-20 as a cartilage patch, and future implantation studies are anticipated. Copyright © 2010 Society of Chemical Industry