Trimethyltin-evoked apoptosis of murine hippocampal granule neurons is accompanied by the expression of interleukin-1beta and interleukin-1 receptor antagonist in cells of ameboid phenotype, the majority of which are NG2-positive
Trimethyltin-evoked apoptosis of murine hippocampal granule neurons is accompanied by the expression of interleukin-1beta and interleukin-1 receptor antagonist in cells of ameboid phenotype, the majority of which are NG2-positive
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DOI：
10.1016/j.brainresbull.2008.02.029
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发表时间：
2008-09-05
影响因子：
3.8
通讯作者：
Oderfeld-Nowak, Barbara
中科院分区：
文献类型：
--
作者：
Fiedorowicz, Anna;Figiel, Izabela;Oderfeld-Nowak, Barbara
白细胞介素-1 β（IL-1 β）与多种神经病理学有关，而IL-1受体拮抗剂（IL-1 ra）已被证明可减少神经元损伤。我们研究了三甲基锡（TMT）中毒后小鼠海马中两种细胞因子的表达模式。使用核糖核酸酶保护试验，我们证明了TMT处理后3天IL-1 β和IL-1 ra的转录诱导与神经元凋亡的峰值相关。在这个时候，免疫细胞化学染色显示NG 2蛋白聚糖表达阿米巴样细胞位于神经毒性损伤的网站，其中一些绑定的小胶质细胞标记物，凝集素的两种细胞因子的表达增强。NG 2和凝集素染色之间存在一些重叠。我们的研究结果表明，这两种细胞因子参与了齿状颗粒细胞的凋亡过程，并表明IL-1 β的促凋亡作用优于IL-1 ra的假定保护作用。这两种细胞因子在阿米巴样表型的NG 2（+）细胞中表达的新发现表明，这些细胞通过促炎和抗炎细胞因子的调节作用，可能参与控制损伤后的神经元死亡或存活。(c)2008年爱思唯尔公司所有的战斗保留。
Interleukin-1beta (IL-1 beta) has been implicated in various neuropathologies, while IL-1 receptor antagonist (IL-1ra) has been shown to reduce neuronal injury. We investigated the pattern of expression of both cytokines in murine hippocampus after trimethyltin (TMT) intoxication. Using a ribonuclease protection assay, we demonstrated induction of transcription of IL-1 beta and IL-1ra 3 days following TMT treatment which correlated with the peak of neuronal apoptosis. At this time, immunocytochemical staining revealed enhanced expression of both cytokines in NG2 proteoglycan expressing ameboid cells located at the site of neurotoxic insult, some of which bound also the microglial marker, lectin. There was some overlap between NG2 and lectin staining. Our results suggest that the two cytokines are involved in apoptotic processes in dentate granule cells and indicate that the pro-apoptotic effect of IL-1 beta prevails over the presumed protective action of IL-1 ra. The novel finding of expression of both cytokines in NG2(+) cells of ameboid phenotype indicates that these cells, through the regulatory roles of pro- and anti-inflammatory cytokines, may be involved in control of neuronal death or survival after injury. (c) 2008 Elsevier Inc. All fights reserved.