Effect of high-intensity focused ultrasound on whole blood with and without microbubble contrast agent
Effect of high-intensity focused ultrasound on whole blood with and without microbubble contrast agent
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DOI：
10.1016/s0301-5629(99)00043-5
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发表时间：
1999-07-01
影响因子：
2.9
通讯作者：
Crum, LA
中科院分区：
文献类型：
--
作者：
Poliachik, SL;Chandler, WL;Crum, LA
使用有和没有造影剂（CA）的人全血样本，我们评估了暴露于聚焦连续波（CW）1.1 MHz超声的影响，持续时间为10 ms至1 s，空间平均强度为560至2360 W/cm（2）。用被动空化检测器监测空化，用光谱法测定溶血。仅在全血中，在任何暴露条件下均未检测到显著的空化、加热或溶血。相反，在CA的全血中检测到空化和溶血，但未检测到加热。CA浓度低至0.28 μ L CA/mt全血，强度为2360 W/cm（2），持续1 s，导致可测量的空化和溶血增加6倍，与假手术相比。空化和溶血增加成比例的CA的浓度和暴露的时间。在每毫升含4.2 μ L CA的样品中，全血暴露1秒，在1750 W/cm（2）处观察到阈值，与较低强度的暴露相比，空化和溶血增加了10倍。含CA的全血的HIFU暴露导致体外显著溶血，并且可能导致体内临床显著溶血。(C)1999年世界医学和生物学超声联合会。
Using human whole blood samples with and without contrast agent (CA), we evaluated the effect of exposures to focused, continuous wave (CW) 1.1-MHz ultrasound for durations of 10 ms to 1 s at spatial average intensities of 560 to 2360 W/cm(2). Cavitation was monitored with a passive cavitation detector and hemolysis was determined with spectroscopy. In whole blood alone, no significant cavitation, heating or hemolysis was detected at any exposure condition. Conversely, cavitation and hemolysis, but not heating, were detected in whole blood with CA. A CA concentration as low as 0.28 mu L CA per mt whole blood at an intensity of 2360 W/cm(2) for 1 s resulted in measurable cavitation and a 6-fold increase in hemolysis compared to shams. Cavitation and hemolysis increased proportional to the concentration of CA and duration of exposure. In samples containing 4.2 mu L CA per mi, whole blood exposed for 1 s, a threshold was seen at 1750 W/cm(2) where cavitation and hemolysis increased IO-fold compared to exposures at lower intensities. HIFU exposure of whole blood containing CA leads to significant hemolysis in vitro and may lead to clinically significant hemolysis in vivo. (C) 1999 World Federation for Ultrasound in Medicine & Biology.