A homologue gene of β-catenin participates in the development of shrimps and immune response to bacteria and viruses
A homologue gene of β-catenin participates in the development of shrimps and immune response to bacteria and viruses
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β-连环蛋白的同源基因参与虾的发育以及对细菌和病毒的免疫反应
DOI：
10.1016/j.fsi.2015.08.030
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发表时间：
2015
影响因子：
4.7
通讯作者：
Cui-Jie Kang
中科院分区：
文献类型：
--
作者：
Ya-Kai Xie;Ding Ding;Hui-Min Wang;Cui-Jie Kang
β-连环蛋白是一种多功能蛋白，参与许多生理过程，包括发育、细胞增殖、细胞迁移和细胞凋亡。然而，β- catenin在甲壳类动物中的作用尚不清楚。本研究首次在日本有袋绦虫（marsupenaeus japonicus，即marsupenaeus）中发现β-cateninin对虾同源基因。Mjβ-catenin)进行了鉴定和表征。mj - β-cateninis互补DNA全长3130 bp，包括一个2463 bp的开放阅读框，编码821个氨基酸。β- catenin蛋白的多重比对表明，Armadillo/β-Catenin-like重复结构域是保守的。系统发育分析表明，虾类β- catenin与无脊椎动物β- catenin聚为一类。在对虾发育的各个阶段均检测到β-连环蛋白的转录，并随着对虾的成熟而持续增加。在成虾中，β-catenin广泛分布于检测组织中，在鳃、血细胞、睾丸和卵巢中表达量较高。在金黄色葡萄球菌、鳗弧菌和白斑综合征病毒感染后，成虾组织中β-连环蛋白转录量在不同时间点均显著上调。此外，β-连环蛋白的敲低导致虾体内细菌清除能力受损和病毒复制增加。因此，虾体内的β-Catenin参与了虾的发育和免疫应答。
β-Catenin is a multifunctional protein that is involved in many physiological processes, including development, cell proliferation, cell migration, and apoptosis. However, the function ofβ-Catenin in crustacean is unknown. In this study, the first shrimp homologous gene ofβ-catenininMarsupenaeus japonicus(i.e.,Mjβ-catenin) was identified and characterized. The full-length of the complementary DNA ofMjβ-cateninis 3130 bp, including a 2463 bp open reading frame that encodes 821 amino acid. Multiple alignment ofβ-Catenin proteins suggested that the Armadillo/β-Catenin-like repeat domains were conserved. Phylogenetic analysis showed thatβ-Catenin from shrimp was clustered into one group with invertebrateβ-Catenin. The transcription ofβ-cateninin various development stages of shrimp was detected and persistently increased as the shrimp matured. In adult shrimp,β-cateninwas widely distributed in detected tissues and has the relatively high expression level in gills, hemocytes, testes, and ovaries. The transcripts ofβ-cateninin tissues of adult shrimp were significantly up-regulated at various time points after infecting withStaphylococcus aureus,Vibrio anguillarum, and white-spot syndrome virus. Furthermore, knockdown ofβ-cateninresulted in impaired bacterial clearance ability and increased virus copy in shrimpin vivo. Therefore,β-Catenin in shrimp participates in the development and immune response of shrimps.