Genetic mapping of highly versatile and solvent-tolerant Pseudomonas putida B6-2 (ATCC BAA-2545) as a 'superstar' for mineralization of PAHs and dioxin-like compounds.
Genetic mapping of highly versatile and solvent-tolerant Pseudomonas putida B6-2 (ATCC BAA-2545) as a 'superstar' for mineralization of PAHs and dioxin-like compounds.
复制标题
高度通用且耐溶剂的恶臭假单胞菌 B6-2 (ATCC BAA-2545) 的遗传图谱，作为多环芳烃和二恶英类化合物矿化的“超级明星”。
DOI：
10.1111/1462-2920.15613
复制
发表时间：
2021-05
影响因子：
5.1
通讯作者：
Hongzhi Tang
中科院分区：
文献类型：
--
作者：
Weiwei Wang;Qinggang Li;Lige Zhang;Jie Cui;Hao Yu;Xiaoyu Wang;Xingyu Ouyang;Fei Tao;Ping Xu;Hongzhi Tang
多环芳烃（PAHs）和二恶英类化合物是一类广泛存在的有毒环境污染物。能够与芳香多环化合物一起生长的各种微生物对于污染场地的生物修复和地球的碳循环至关重要。在这里，在联苯（BP）存在下生长的恶臭假单胞菌B6-2（ATCC BAA-2545）的细胞能够同时降解多环芳烃及其衍生物，即使它们作为混合物存在，并且耐受高浓度的剧毒溶剂。菌株B6-2的6.37 Mb基因组的遗传分析揭示了负责芳香族化合物的中央分解代谢系统和溶剂耐受性的基因簇的共存。我们使用功能转录组学和蛋白质组学来鉴定与BP以及BP、二苯并呋喃、二苯并噻吨和咔唑的混合物的catabetamine相关的候选基因。此外，我们观察到的动态变化，转录水平与BP，包括在芳香族化合物，趋化性，外排泵，转运蛋白的代谢途径可能参与适应多环芳烃。对菌株B6-2的高度通用活性的研究表明，它是一个潜在的有用的模型，用于污染场地的生物修复和假单胞菌的生化，遗传和进化方面的调查。本文受版权保护。All rights reserved.
Polycyclic aromatic hydrocarbons (PAHs) and dioxin-like compounds, including sulfur, nitrogen and oxygen heterocycles, are widespread and toxic environmental pollutants. A wide variety of microorganisms capable of growing with aromatic polycyclic compounds are essential for bioremediation of the contaminated sites and the Earth's carbon cycle. Here, cells of Pseudomonas putida B6-2 (ATCC BAA-2545) grown in the presence of biphenyl (BP) are able to simultaneously degrade PAHs and their derivatives, even when they are present as mixtures, and tolerate high concentrations of extremely toxic solvents. Genetic analysis of the 6.37 Mb genome of strain B6-2 reveals coexistence of gene clusters responsible for central catabolic systems of aromatic compounds and for solvent tolerance. We used functional transcriptomics and proteomics to identify the candidate genes associated with catabolism of BP and a mixture of BP, dibenzofuran, dibenzothiophene and carbazole. Moreover, we observed dynamic changes in transcriptional levels with BP, including in metabolic pathways of aromatic compounds, chemotaxis, efflux pumps, and transporters potentially involved in adaptation to PAHs. This study on the highly versatile activities of strain B6-2 suggests it to be a potentially useful model for bioremediation of polluted sites and for investigation of biochemical, genetic and evolutionary aspects of Pseudomonas. This article is protected by copyright. All rights reserved.