A Zinc-Dependent Mechanism Regulates Meiotic Progression in Mammalian Oocytes
A Zinc-Dependent Mechanism Regulates Meiotic Progression in Mammalian Oocytes
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DOI：
10.1095/biolreprod.111.097253
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发表时间：
2012-04-01
影响因子：
3.6
通讯作者：
Woodruff, Teresa K.
中科院分区：
文献类型：
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作者：
Bernhardt, Miranda L.;Kong, Betty Y.;Woodruff, Teresa K.
减数分裂过程的精确协调是卵子成功受精能力的关键决定因素，锌在这一过程中已成为关键的调节因素。这种过渡金属调控作用的早期表现是细胞内总锌的显著增加。这种积累对于超过末期I的减数分裂进程和中期II减数分裂停滞的建立是必不可少的。随后的发育事件，受精，诱导不稳定锌的快速排出，这是减数分裂恢复的一个标志性事件。在目前的研究中，我们发现锌通量的部分作用是通过改变细胞抑制因子（CSF）的活性来起作用的，CSF是在成熟的未受精卵中建立和维持中期II停滞所需的细胞活性。我们提出了一个模型，其中锌通过脑脊液成分EMI2（一种锌结合蛋白）对减数分裂进行浓度依赖性调节。综上所述，这些数据支持锌本身通过其与EMI2的相互作用是脑脊液的核心成分的结论。
Precise coordination of meiotic progression is a critical determinant of an egg's capacity to be fertilized successfully, and zinc has emerged as a key regulatory element in this process. An early manifestation of a regulatory role for this transition metal is the significant increase in total intracellular zinc. This accumulation is essential for meiotic progression beyond telophase I and the establishment of meiotic arrest at metaphase II. The subsequent developmental event, fertilization, induces a rapid expulsion of labile zinc that is a hallmark event in meiotic resumption. In the present study, we show that the zinc fluxes work, in part, by altering the activity of the cytostatic factor (CSF), the cellular activity required for the establishment and maintenance of metaphase II arrest in the mature, unfertilized egg. We propose a model in which zinc exerts concentration-dependent regulation of meiosis through the CSF component EMI2, a zinc-binding protein. Together, the data support the conclusion that zinc itself, through its interaction with EMI2, is a central component of the CSF.