Ginkgetin exerts anti-inflammatory effects on cerebral ischemia/reperfusion-induced injury in a rat model via the TLR4/NF-κB signaling pathway
Ginkgetin exerts anti-inflammatory effects on cerebral ischemia/reperfusion-induced injury in a rat model via the TLR4/NF-κB signaling pathway
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DOI：
10.1080/09168451.2018.1553608
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发表时间：
2019-04-03
影响因子：
1.6
通讯作者：
Peng, Xuemei
中科院分区：
文献类型：
--
作者：
Li, Qin;Ye, Tao;Peng, Xuemei
银杏叶是一种从银杏叶中分离出来的天然类双黄酮，据报道具有很强的抗炎和免疫抑制作用。本研究旨在探讨银杏黄酮对脑缺血再灌注（I/R）损伤的抗炎机制。采用免疫组织化学染色、Western blot和酶样免疫吸附法（ELISA）检测脑缺血半球炎症相关细胞因子。我们的研究结果表明，银杏苷治疗可显著恢复大鼠脑I/ r诱导的神经功能缺损评分。诱导型一氧化氮合酶（iNOS）和环氧化酶-2 （COX-2）在银杏素（100 mg/kg）处理组的表达也显著降低。银杏素治疗组炎症相关蛋白前列腺素E2 （PGE2）、肿瘤坏死因子α (tnf - α)、白细胞介素1 β (IL-1 β)、白细胞介素6 （IL-6）、白细胞介素8 （IL-8）的表达均降低。而白细胞介素-10 （IL-10）的表达明显升高。因此，本研究表明银杏苷通过下调促炎细胞因子和阻断TLR4/NF-kappa B通路，保护神经元免受I/ r诱导的大鼠损伤。
Ginkgo biloba, a natural biflavonoid isolated from Ginkgo biloba leaves, is reported to have strong anti-inflammatory and immunosuppressive properties. The aim of this study is to investigate the potential anti-inflammatory mechanisms of ginkgo flavonoids on cerebral ischemia/reperfusion (I/R) injury. Inflammatory-associated cytokines in cerebral ischemic hemispheres were determined by immunohistochemical staining, Western blot and enzyme-like immunosorbent assay (ELISA). Our results indicated that treatment with Ginkgetin significantly restored rat brain I/R-induced neurological deficit scores. Inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression in Ginkgetin treatment group (100 mg/kg) also significantly reduced. The expression inflammation-related protein prostaglandin E2 (PGE2), tumor necrosis factor alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6) and interleukin-8 (IL-8) was also decreased in Ginkgetin treatment group. However, the expression of interleukin-10 (IL-10) was remarkably increased. Thus, this study demonstrates that Ginkgetin protects neurons from I/R-induced rat injury by down-regulating pro-inflammatory cytokines and blocking the TLR4/NF-kappa B pathway.