Development of siRNA for therapeutics: Efficient synthesis of phosphorothioate RNA utilizing phenylacetyl disulfide (PADS)
Development of siRNA for therapeutics: Efficient synthesis of phosphorothioate RNA utilizing phenylacetyl disulfide (PADS)
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DOI：
10.1016/j.bmcl.2006.01.081
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发表时间：
2006-05-01
影响因子：
2.7
通讯作者：
Barone, S
中科院分区：
文献类型：
--
作者：
Ravikumar, VT;Andrade, M;Barone, S
在吡啶和乙腈（1：1，v/v）的混合物中，使用苯乙酰基二硫化物（PADS）3 min，可有效合成硫代磷酸RNA（PS-RNA）。该试剂在含有PS：PO混合骨架的RNA合成过程中也有效。(C)2006爱思唯尔有限公司保留所有权利。
Efficient synthesis of phosphorothioate RNA (PS-RNA) is demonstrated by using phenylacetyl disulfide (PADS) in a mixture of pyridine and acetonitrile (1:1, v/v) for 3 min. Sulfurization is achieved with >99.8% stepwise efficiency. This reagent also performs efficiently during synthesis of RNA containing PS:PO mixed backbone. (C) 2006 Elsevier Ltd. All rights reserved.