GENE FOR HUMAN FACTOR-X - A BLOOD-COAGULATION FACTOR WHOSE GENE ORGANIZATION IS ESSENTIALLY IDENTICAL WITH THAT OF FACTOR-IX AND PROTEIN-C
GENE FOR HUMAN FACTOR-X - A BLOOD-COAGULATION FACTOR WHOSE GENE ORGANIZATION IS ESSENTIALLY IDENTICAL WITH THAT OF FACTOR-IX AND PROTEIN-C
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DOI：
10.1021/bi00366a018
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发表时间：
1986-09-09
期刊：
影响因子：
2.9
通讯作者：
DAVIE, EW
中科院分区：
文献类型：
--
作者：
LEYTUS, SP;FOSTER, DC;DAVIE, EW
因子X是已知参与血液凝固的六种维生素K依赖性蛋白质之一，其他是因子VII、因子IX、凝血酶原、蛋白质S和蛋白质C。在本研究中，重组噬菌体含有重叠的DNA插入编码的基因为人类因子X已被分离和表征。这些DNA插入物编码几乎整个X因子基因，从前前导肽延伸到转录产物的3“非编码区。通过DNA测序确定X因子基因的结构，以确定基因中内含子和外显子的位置。鉴定了7个内含子和8个外显子，并建立了它们的内含子/外显子边界。这七个内含子在氨基酸序列中与人因子IX和蛋白C基因中的内含子基本相同的位置中断编码序列。此外，因子X基因中的内含子将编码序列分成离散的外显子，这些外显子编码蛋白质的潜在结构和功能结构域。这一信息提供了强有力的证据，支持这样的建议，即存在于血浆中的维生素K依赖性蛋白质是从一个单一的共同基因进化而来的，并且这个祖先基因是通过一个过程产生的，该过程涉及将DNA的小蛋白质编码单位组装成一个单一的基因。
Factor X is one of six vitamin K dependent proteins known to be involved in blood coagulation, the others being factor VII, factor IX, prothrombin, protein S, and protein C. In the present studies, recombinant bacateriophage containing overlapping DNA inserts coding for the gene for human factor X have been isolated and characterized. These DNA inserts code for almost the entire gene for factor X, extending from the prepro leader peptide through the 3'' noncoding region of the transcription product. The organization of the gene for factor X was established by DNA sequencing to identify the location of the introns and exons in the gene. Seven introns and eight exons were identified and their intron/exon boundaries established. The seven introns interrupt the coding sequence at essentially identical locations in the amino acid sequence as the introns in the genes for human factor IX and protein C. In addition, the introns in the gene for factor X divide the coding sequence into discrete exons that code for potential structural and functional domains of the protein. This information provides strong evidence to support the suggestion that the vitamin K dependent proteins present in plasma have evolved from a single, common gene and that this ancestral gene arose through a process that involved the assembly of small protein coding units of DNA into a single gene.