Cholinergic innervation of fetal neocortical transplants is increased after neutralization of myelin-associated neurite growth inhibitors.
Cholinergic innervation of fetal neocortical transplants is increased after neutralization of myelin-associated neurite growth inhibitors.
复制标题
中和髓磷脂相关神经突生长抑制剂后，胎儿新皮质移植物的胆碱能神经支配增加。
DOI：
10.1006/exnr.1997.6731
复制
发表时间：
1998
影响因子：
5.3
通讯作者：
Kartje,GL
中科院分区：
文献类型：
--
作者：
Schulz,MK;Schnell,L;Castro,AJ;Schwab,ME;Kartje,GL
已知的是，将胎儿新皮质移植到成年新皮质感觉运动性吸入性损伤中，可以从成年宿主大鼠的大脑接受传入输入。由于这种输入的密度低于正常，本研究旨在研究髓鞘相关轴突生长抑制剂NI-35/250中和是否可能促进宿主来源的胆碱能神经在胎儿新皮质移植中的支配。成年大鼠接受单侧感觉运动性皮质抽吸损伤，胚胎14-15天的新皮质组织块立即植入病变腔内。小鼠杂交瘤细胞分泌阻断神经突起生长抑制剂NI-35/250的单抗IN-1，或对照抗体或不含细胞的培养液，直接应用于胎儿移植物组织上方的米氏滤膜胶囊中。12周后，对大脑进行处理，以显示乙酰胆碱酯酶阳性的假定胆碱能纤维。我们发现，在接受IN-1抗体治疗的受者中，胎儿移植物的胆碱能神经支配在移植物内生长的纤维和移植物中心内的密度方面都得到了增强。这些结果表明，髓鞘相关的轴突生长抑制物参与了成人大脑中宿主-移植连接性的发展。
Fetal neocortical transplants placed into adult neocortical sensorimotor aspiration lesions are known to receive afferent input from the adult host rat brain. As this input is less dense than normal, the present study was designed to investigate whether neutralization of myelin-associated neurite growth inhibitors NI-35/250 might promote host derived cholinergic innervation of fetal neocortical transplants. Adult rats received unilateral sensorimotor cortical aspiration lesions, and block grafts from embryonic day 14–15 neocortical tissue were placed immediately into the lesion cavities. Mouse hybridoma cells secreting either the monoclonal antibody IN-1, which blocks neurite growth inhibitors NI-35/250, or a control antibody or medium without cells were applied in millipore filter capsules directly over the fetal graft tissue. The brains were processed 12 weeks later for the visualization of acetylcholinesterase-positive, presumptive cholinergic fibers. We found an enhancement in the cholinergic innervation of fetal grafts in the recipients treated with the antibody IN-1 both in terms of fibers growing into the graft and of density within the center of the grafts. These results indicate that myelin-associated neurite growth inhibitors are involved in the development of host–transplant connectivity in the adult brain.