Covalent microcontact printing of proteins for cell patterning
Covalent microcontact printing of proteins for cell patterning
复制标题
DOI：
10.1002/chem.200501554
复制
发表时间：
2006-08-16
影响因子：
4.3
通讯作者：
Reinhoudt, David N.
中科院分区：
文献类型：
--
作者：
Rozkiewicz, Dorota I.;Kraan, Yvonne;Reinhoudt, David N.
我们描述了一种直接的方法，通过微接触印刷的共价固定的嗜细胞蛋白，它可以用来图案的底物上的细胞。利用亚胺化学将嗜细胞蛋白以微图案印刷在反应性自组装单层上。氨基端单分子膜与对苯二醛反应，在玻璃或金上得到了端醛单分子膜。利用氧化聚二甲基硅氧烷（PDMS）印迹，将醛单层用作生物工程类胶原蛋白直接微接触打印的底物。在将蛋白质固定成粘附的“岛”后，剩余的区域被氨基聚乙二醇阻断，形成一层抵抗细胞粘附的层。人恶性癌（HeLa）细胞被播种并孵育到有图案的底物上。研究发现，这些细胞选择性地附着和扩散在蛋白岛上，并避开聚乙二醇（PEG）区。这些发现说明了微接触印刷作为一种定位表面蛋白质的方法的重要性，并展示了受控表面化学指导细胞粘附的范围。
We describe a straightforward approach to the covalent immobilization of cytophilic proteins by micro-contact printing, which can be used to pattern cells on substrates. Cytophilic proteins are printed in micropatterns on reactive self-assembled monolayers by using imine chemistry. An aldehyde-terminated monolayer on glass or on gold was obtained by the reaction between an amino-terminated monolayer and terephthaldialdehyde. The aldehyde monolayer was employed as a substrate for the direct microcontact printing of bioengineered, collagen-like proteins by using an oxidized poly(dimethylsiloxane) (PDMS) stamp. After immobilization of the proteins into adhesive "islands", the remaining areas were blocked with amino-poly(ethylene glycol), which forms a layer that is resistant to cell adhesion. Human malignant carcinoma (HeLa) cells were seeded and incubated onto the patterned substrate. It was found that these cells adhere to and spread selectively on the protein islands, and avoid the poly(ethylene glycol) (PEG) zones. These findings illustrate the importance of microcontact printing as a method for positioning proteins at surfaces and demonstrate the scope of controlled surface chemistry to direct cell adhesion.