Oxidative events during programmed cell death of daylily (Hemerocallis hybrid) petals
Oxidative events during programmed cell death of daylily (Hemerocallis hybrid) petals
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DOI：
10.1016/s0168-9452(98)00034-x
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发表时间：
1998-04-24
期刊：
影响因子：
5.2
通讯作者：
Rubinstein, B
中科院分区：
文献类型：
--
作者：
Panavas, T;Rubinstein, B
黄花菜（Hemerocallis 杂交品种 Stella d'Oro）的花瓣在开花后仅 24 小时就显示出明显的退化迹象。在此之前，离子泄漏会增加。由于膜差异渗透性的损失与活性氧 (ROS) 有关，我们想知道黄花菜衰老是否部分受到与 ROS 相关的反应的控制。低氧条件可防止离子泄漏，甚至在开花前过氧化脂质、脂氧合酶活性和 H2O2 都会增加。刺激细胞死亡的治疗也会过早地增加 H2O2 水平。在花朵衰老过程中，过氧化氢酶和抗坏血酸过氧化物酶等保护性酶的比活性会降低，抗坏血酸水平也会降低。超氧化物歧化酶和过氧化物酶比活性增加。我们认为，导致细胞死亡的膜变化可能部分是由脂氧合酶活性和由于某些保护性酶的有效性降低而增加的 ROS 引起的。 (C) 1998 Elsevier Science Ireland Ltd. 保留所有权利。
Petals of the daylily, Hemerocallis hybrid cv Stella d'Oro, show visible signs of degradation just 24 h after flower opening. This is preceded by increases in ion leakage. Since losses of membrane differential permeability have been linked to reactive oxygen species (ROS), we are asking if daylily senescence is controlled in part by reactions associated with ROS. Ion leakage is prevented by hypoxic conditions, and peroxidized lipid, lipoxygenase activity and H2O2 increase even before flower opening. Treatments that stimulate cell death also prematurely increase levels of H2O2. The specific activity of protective enzymes such as catalase and ascorbate peroxidase decreases during flower senescence as do levels of ascorbic acid. Superoxide dismutase and peroxidase specific activities increase. We suggest that membrane changes leading to cell death may be induced in part by lipoxygenase activity and by ROS that are increasing because of reduced effectiveness of certain protective enzymes. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.