Effects of magnesium sulfate on apoptosis in cultured human gastric epithelial cells
Effects of magnesium sulfate on apoptosis in cultured human gastric epithelial cells
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硫酸镁对培养人胃上皮细胞凋亡的影响
DOI：
10.1080/09540105.2015.1079596
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发表时间：
2016
影响因子：
3
通讯作者：
Sun Juan
中科院分区：
文献类型：
--
作者：
Xia Yuan;Bo Agula;Liu Zhiyue;Chi Baofeng;Su Zhuang;Hu Yueling;Luo Ru;Su Xiong;Sun Juan
本研究探讨了硫酸镁（MgSO 4）对人胃腺癌（AGS）上皮细胞株活力的影响。AGS细胞暴露于不同浓度的MgSO 4不同的时间段和细胞活力的影响，DNA片段，凋亡基因的表达，和半胱天冬酶活性进行了研究。在这项研究中采用了一系列的测试，包括乳酸脱氢酶细胞活力测定，膜联蛋白V染色，逆转录聚合酶链反应（RT-PCR），末端脱氧核苷酸转移酶介导的dUTP缺口末端标记（TUNEL），和几个caspase活性测定。RT-PCR检测肿瘤坏死因子相关凋亡诱导配体（TRAIL）、Fas配体（FasL）和Fas受体（FasL）的表达。这些分析表明，硫酸镁诱导AGS细胞活力的剂量依赖性降低。膜联蛋白V染色在阴性对照和处理的细胞之间没有差异，表明没有检测到凋亡细胞。TUNEL检测结果与Annexin V染色结果一致。MgSO 4可剂量依赖性地增加FasL mRNA的表达，降低FasL mRNA的表达。MgSO 4处理倾向于引起caspase-3、-6、-8和-9活性的初始（1 h）剂量依赖性增加;然而，这些活性随后被抑制（24 h）。 我们推测MgSO 4通过激活caspase对AGS细胞产生抗凋亡作用。
This study investigated the effect of magnesium sulfate (MgSO4) on the viability of the human gastric adenocarcinoma (AGS) epithelial cell line. AGS cells were exposed to various concentrations of MgSO4for different time-periods and the effects on cell viability, DNA fragmentation, apoptotic gene expression, and caspase activity were investigated. A range of tests were employed in this study, including a lactate dehydrogenase cell viability assay, annexin V staining, reverse transcription polymerase chain reaction (RT-PCR), terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL), and several caspase activity assays. Expression of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), Fas ligand (FasL) and Fas receptor (FasR) were analyzed using RT-PCR. These analyses revealed that MgSO4induced a dose-dependent reduction in AGS cell viability. Annexin V staining did not differ between negative control and treated cells, indicating that no apoptotic cells were detected. The TUNEL assay data were consistent with the annexin V staining results. FasL mRNA expression was increased and FasR mRNA expression was decreased in a dose-dependent manner by MgSO4. MgSO4treatment tended to cause an initial (1 h) dose-dependent increase in the activities of caspase-3, -6, -8, and -9; however, these activities were subsequently inhibited (24 h). We deduced that MgSO4exerted an anti-apoptotic effect in AGS cells via caspase activation.