Comparative analysis of rosaceous genomes and the reconstruction of a putative ancestral genome for the family.
Comparative analysis of rosaceous genomes and the reconstruction of a putative ancestral genome for the family.
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DOI：
10.1186/1471-2148-11-9
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发表时间：
2011-01-12
影响因子：
3.4
通讯作者：
Troggio M
中科院分区：
文献类型：
--
作者：
Illa E;Sargent DJ;Lopez Girona E;Bushakra J;Cestaro A;Crowhurst R;Pindo M;Cabrera A;van der Knaap E;Iezzoni A;Gardiner S;Velasco R;Arús P;Chagné D;Troggio M
迄今为止，在蔷薇科进行的比较基因组图谱研究是通过对同一属或密切相关属的遗传图谱进行比对，并使用有限数量的共同标记。随着Prunus和Fragaria基因组资源和序列数据的不断增长，可以将这两个属与最近公布的Malus × domestica基因组序列进行详细的比较。我们使用806个分子标记进行了比较分析，这些分子标记在遗传上锚定在Prunus和/或Fragaria参考图谱上，在物理上锚定在苹果基因组序列上。苹果和李属、苹果和花属的共有标记分别为784个和148个。在蔷薇科3属中，标记位置之间具有较高的对应性，并存在保守的共块。我们重建了玫瑰科的祖先基因组。包含9条染色体的基因组最有可能是蔷薇科祖先的祖先。所调查的三个属之间观察到的染色体易位数量很低。然而，在苹果和李子中发现的倒置数量远高于任何报道的植物基因组比较，这表明小的倒置在蔷薇科这两个属的进化中发挥了重要作用。
Comparative genome mapping studies in Rosaceae have been conducted until now by aligning genetic maps within the same genus, or closely related genera and using a limited number of common markers. The growing body of genomics resources and sequence data for both Prunus and Fragaria permits detailed comparisons between these genera and the recently released Malus × domestica genome sequence. We generated a comparative analysis using 806 molecular markers that are anchored genetically to the Prunus and/or Fragaria reference maps, and physically to the Malus genome sequence. Markers in common for Malus and Prunus, and Malus and Fragaria, respectively were 784 and 148. The correspondence between marker positions was high and conserved syntenic blocks were identified among the three genera in the Rosaceae. We reconstructed a proposed ancestral genome for the Rosaceae. A genome containing nine chromosomes is the most likely candidate for the ancestral Rosaceae progenitor. The number of chromosomal translocations observed between the three genera investigated was low. However, the number of inversions identified among Malus and Prunus was much higher than any reported genome comparisons in plants, suggesting that small inversions have played an important role in the evolution of these two genera or of the Rosaceae.