GLIOBLASTOMA CELLS DO NOT INTRAVASATE INTO BLOOD-VESSELS
GLIOBLASTOMA CELLS DO NOT INTRAVASATE INTO BLOOD-VESSELS
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DOI：
10.1227/00006123-199501000-00016
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发表时间：
1995-01-01
期刊：
影响因子：
4.8
通讯作者：
WOODARD, CA
中科院分区：
文献类型：
--
作者：
BERNSTEIN, JJ;WOODARD, CA
胶质母细胞瘤在中枢神经系统外很少发现。使用大鼠同种移植模型和两种体外模型测试大鼠C6胶质母细胞瘤细胞向中枢神经系统和软脑膜血管内渗的能力。在体内，扫描电子显微镜表明，移植C6细胞植入口袋中的大鼠皮质，血管可以幸免于在宿主脑实质中形成的大消化囊肿。移植的大鼠皮质的免疫细胞化学显示胶质母细胞瘤细胞位于血管基底膜上，被细胞外基质材料纤连蛋白包围。血管内皮细胞内层粘连蛋白和纤维连接蛋白，并有内皮细胞增生的地区。血管内未观察到C6细胞。在体外，与新鲜大鼠软脑膜血管接种的C6细胞培养物表现出与其他模型中相同的C6胶质母细胞瘤细胞与血管的关系。C6细胞在软脑膜血管基底膜上迁移，但不渗入血管。为了确定血管基底膜的结构和组分是否是胶质母细胞瘤细胞从血管中排除的重要因素，将C6细胞接种在人工基底膜水合凝胶晶片上。接种后1天，C6细胞迁移到人工基底膜凝胶片中。这些数据表明，胶质母细胞瘤细胞被限制在中枢神经系统无法通过重要的基底膜。
GLIOBLASTOMA IS VERY rarely found outside the central nervous system. The ability of rat C6 glioblastoma cells to intravasate into central nervous system and pial blood vessels is tested using a rat homografting model and two in vitro models. In vivo, scanning electron microscopy demonstrates that upon grafting C6 cells into implantation pockets in rat cortex, blood vessels can be spared in large digestion cysts formed in host brain parenchyma. Immunocytochemistry of the grafted rat cortex reveals that the glioblastoma cells are upon the blood vessel basement membrane, surrounded by the extracellular matrix material, fibronectin. The endothelial cells of the blood vessel are inside the laminin and fibronectin, and there were areas of endothelial cell hyperplasia. C6 cells are not observed inside blood vessels. In vitro, C6 cell cultures seeded with blood vessels from fresh rat pia exhibit the same relationship of the C6 glioblastoma cells to the blood vessel as those in the other models. The C6 cells migrate upon the pial blood vessel basement membrane but do not intravasate into the blood vessel. To ascertain whether structure and components of the blood vessel basement membrane are important factors in glioblastoma cell exclusion from blood vessels, C6 cells are seeded upon artificial basement membrane hydrated gel wafers. C6 cells migrate into the artificial basement membrane gel wafer by 1 day after seeding. These data indicate that glioblastoma cells are confined to the central nervous system by an inability to pass through vital basement membrane.