MIGRATION SEDIMENTATION TECHNIQUE AS A PREDICTIVE TEST FOR THE FERTILIZING-CAPACITY OF SPERMATOZOA IN AN INVITRO FERTILIZATION PROGRAM
MIGRATION SEDIMENTATION TECHNIQUE AS A PREDICTIVE TEST FOR THE FERTILIZING-CAPACITY OF SPERMATOZOA IN AN INVITRO FERTILIZATION PROGRAM
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DOI：
10.1111/j.1365-2605.1992.tb01143.x
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发表时间：
1992-12-01
影响因子：
--
通讯作者：
YOGEV, L
中科院分区：
文献类型：
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作者：
HAUSER, R;HOMONNAI, ZT;YOGEV, L
迁移沉降技术（MST）已被提议作为分离高质量活动精子的一种手段。本研究的目的是评估 MST 分离后精子的表现是否可以预测其在体外受精 (IVF) 计划中的受精能力。对 90 份精液样本进行了分析，用于 IVF 胚胎移植 (ET) 计划。每个样本被分为两部分：一部分在 IVF 程序中进行处理，并在精子游上分离后用于人类卵子的授精。另一部分（0.2 毫升）通过 MST 分离，然后对精子的浓度、活力、活力程度和形态进行表征。然后将 MST 分离后的精子特征与 IVF 受精率结果相关联。在 90 个 IVF-ET 周期中的 79 个周期中，至少有一个卵母细胞受精。与没有受精的 IVF 周期相比，受精周期中所有 MST 后的精子特征均显着更高。与未受精的精液样本相比，MST 后受精的精液样本中回收的总活动精子比例更高（分别为 39.9 +/- 3.6 和 20.6 +/- 6.6%；P
The migration-sedimentation technique (MST) has been proposed as a means of separating high quality motile spermatozoa. The present study was conducted in order to evaluate whether sperm performance following separation by MST predicts their fertilizing capacity in an in-vitro fertilization (IVF) programme. Ninety semen specimens were analysed for use in an IVF-embryo transfer (ET) programme. Each specimen was divided into two parts: one was processed in the IVF programme and was used after sperm swim-up separation for insemination of human ova. The other aliquot (0.2 ml) was separated by MST, and the sperm then characterized by their concentration, motility, degree of motility and morphology. Sperm characteristics after separation by MST were then correlated with the results of the IVF-fertilization rates. In 79 of 90 IVF-ET cycles, at least one oocyte was fertilized. All post-MST sperm characteristics were significantly higher in cycles with fertilizations compared to IVF cycles without fertilization. A larger percentage of the total motile spermatozoa were recovered after MST in semen specimens with fertilization, compared to semen specimens without fertilization (39.9 +/- 3.6 and 20.6 +/- 6.6%, respectively; P