Paving the way for transgenic schistosomes
Paving the way for transgenic schistosomes
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DOI：
10.1017/s0031182011001466
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发表时间：
2012-04-01
期刊：
影响因子：
2.4
通讯作者：
Grevelding, C. G.
中科院分区：
文献类型：
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作者：
Beckmann, S.;Grevelding, C. G.
在寄生虫学研究中，基因组学和转录组学已经取得了显著的进展，但用于功能基因分析的转基因系统主要限于原生动物领域。基因插入和敲除策略可以应用于寄生原生动物以及通过RNA干扰（RNAi）进行基因沉默。相比之下，对寄生蠕虫的研究仍然滞后。沿着基因组和转录组分析的重大进展。G.对于染色体，对感兴趣的基因进行功能表征的方法仍处于初始阶段，现在必须在后基因组时代开始时加以详细阐述。在这篇综述中，我们将总结在过去的十年中所作的尝试，建立协议，以瞬时和稳定的转化或抑制染色体。除了使用粒子轰击、电穿孔或基于病毒的感染策略将DNA构建体引入成虫和幼虫幼虫期以表达报告基因的方法之外，还进行了建立基于浸泡、脂质转染和/或电穿孔的RNA干扰方案以沉默基因活性的第一种方法。虽然在这些情况下可以看到显着的进展，但染色体群体急切地等待重大突破，特别是在稳定转化方面，而且还需要沉默或敲除每个感兴趣的染色体基因的策略。
In parasitological research, significant progress has been made with respect to genomics and transcriptomics but transgenic systems for functional gene analyses are mainly restricted to the protozoan field. Gene insertion and knockout strategies can be applied to parasitic protozoa as well as gene silencing by RNA interference (RNAi). By contrast, research on parasitic helminthes still lags behind. Along with the major advances in genome and transcriptome analyses e. g. for schistosomes, methods for the functional characterization of genes of interest are still in their initial phase and have to be elaborated now, at the beginning of the post-genomic era. In this review we will summarize attempts made in the last decade regarding the establishment of protocols to transiently and stably transform or transfect schistosomes. Besides approaches using particle bombardment, electroporation or virus-based infection strategies to introduce DNA constructs into adult and larval schistosome stages to express reporter genes, first approaches have also been made in establishing protocols based on soaking, lipofection, and/or electroporation for RNA interference to silence gene activity. Although in these cases remarkable progress can be seen, the schistosome community eagerly awaits major breakthroughs especially with respect to stable transformation, but also for silencing or knock-down strategies for every schistosome gene of interest.